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101406 VRB (Violet Red Bile Lactose) agar

101406
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

ph ValueSolubility
7.3 - 7.5 (40 g/l, H₂O, 37 °C) (after autoclaving)39.5 g/l

Products

Catalogue NumberPackaging Qty/Pack
1014060500 Plastic bottle 500 g
1014065000 Plastic drum 5 kg
Description
Catalogue Number101406
Replaces1014065007
SynonymsCrystal-violet neutral red bile agar, VRB-agar (Crystal-violet neutral-red bile agar)
OverviewGranuCult™ is our new brand name for our existing granulated dehydrated culture media which apply to new EN ISO 11133:2014 and various other ISO standards and FDA-BAM / USDA-FSIS methods for testing of food and water samples. All these media are currently being updated to be fully compliant to the latest version of these standards and regulations with respect to composition and quality control and will be available shortly.
References
Product Information
HS Code3821 00 00
Quality LevelMQ200
Applications
Biological Information
Physicochemical Information
pH value7.3 - 7.5 (40 g/l, H₂O, 37 °C) (after autoclaving)
Bulk density670 kg/m3
Solubility39.5 g/l
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Storage class10 - 13 Other liquids and solids
WGKWGK 3 highly hazardous to water
Disposal3
Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.
Safety Information
Product Usage Statements
Storage and Shipping Information
StorageStore at +15°C to +25°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Appearance (clearness)clear
Appearance (color)red
pH-value (25 °C)7.2 - 7.6
Solidification behaviour (2 hrs., 45 °C)liquid
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012))
Colony count (Escherichia coli ATCC 8739 (WDCM 00012))
Recovery on test medium (Escherichia coli ATCC 8739 (WDCM 00012))≥ 50 %
Colony color (Escherichia coli ATCC 8739 (WDCM 00012))purplish-red colonies with or without precipitate halo
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013))
Colony count (Escherichia coli ATCC 25922 (WDCM 00013))
Recovery on test medium (Escherichia coli ATCC 25922 (WDCM 00013))≥ 50 %
Colony color (Escherichia coli ATCC 25922 (WDCM 00013))purplish-red colonies with or without precipitate halo
Inoculum on reference medium (Enterobacter cloacae ATCC 13047 (WDCM 00083))
Colony count (Enterobacter cloacae ATCC 13047 (WDCM 00083))
Recovery on test medium (Enterobacter cloacae ATCC 13047 (WDCM 00083))≥ 50 %
Colony color (Enterobacter cloacae ATCC 13047 (WDCM 00083))purplish-red colonies with or without precipitate halo
Growth (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025))no limit
Colony color (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025))colourless to beige colonies
Growth (Enterococcus faecalis ATCC 19433 (WDCM 00009))total inhibition
Growth (Enterococcus faecalis ATCC 29212 (WDCM 00087))total inhibition
Incubation: 24 ± 2 hours at 30 ± 1 °C
Reference media: Tryptic Soy Agar
A recovery rate of 50 % is equivalent to a productivity value of 0.5.
The indicated colony counts result from the sum of a triple determination.
Global Trade Item Number
Catalogue Number GTIN
1014060500 04022536020310
1014065000 04022536020341

Documentation

VRB (Violet Red Bile Lactose) agar SDS

Title

Safety Data Sheet (SDS) 

VRB (Violet Red Bile Lactose) agar Certificates of Analysis

Product Number PackagingSpecificationLot Number
1014060500 Plastic bottlePDF Specification Document
1014065000 Plastic drumPDF Specification Document

Brochure

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Categories

Industrial Microbiology > Bioburden Testing > Microbiological Water Testing > Microbiological Water Testing for Hospital & Clinical
Industrial Microbiology > Culture Media > Culture Media for Food & Beverage Industry > Dehydrated Culture Media
Industrial Microbiology > Culture Media > Culture Media for Food & Beverage Industry > Dehydrated Culture Media > Enrichment, Isolation & Differentiation by Organism > Coliforms, E. coli & Enterobacteriaceae
Industrial Microbiology > Bioburden Testing > Microbiological Water Testing > Microbiological Water Testing for Pharmaceutical & Cosmetics Industry
Industrial Microbiology > Pathogen & Spoilage Testing > Pathogen Detection > Pathogen Detection by Product > Dehydrated and Ready-to-use Culture Media
Industrial Microbiology > Bioburden Testing > Microbiological Water Testing > Microbiological Water Testing for Food & Beverage Industry > Cultivation
Industrial Microbiology > Bioburden Testing > Microbiological Water Testing > Water Testing for Municipal & Environmental Water
Industrial Microbiology > Pathogen & Spoilage Testing > Pathogen Detection > Pathogen Detection by Organism > Clostridium Perfringens
Industrial Microbiology > Pathogen & Spoilage Testing > Pathogen Detection > Pathogen Detection by Organism > E. Coli O157 & STEC
Industrial Microbiology > Culture Media > Culture Media for Pharmaceutical & Cosmetics Industry > Dehydrated Culture Media > Isolation & Differentiation by Organism > Coliforms, E. coli & Enterobacteriaceae
Industrial Microbiology > Culture Media > Culture Media for Food & Beverage Industry > Dehydrated Culture Media > Enrichment, Isolation & Differentiation by Organism > Clostridia & Sulfite reducing anaerobic Bacteria