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654161 Tubulin-α-1, His•Tag® Fusion, E. coli

654161
  
Purchase on Sigma-Aldrich

Overview

Description
Overview

This product has been discontinued.





Full-length, recombinant, human tubulin-α-1 (TBA1, TUBA1, α-tubulin 1) fused to HisTag® and STag™ sequences at the N-terminus and expressed in E. coli. This preparation is qualified for use as a substrate for protein tyrosine kinases in in vitro assays. Tubulin-α-1 is a major constituent of microtubules. It binds one GTP at a nonexchangeable site (aa 142-148) on the alpha-chain. IFN-α-induced phosphorylation at Tyr271 has been demonstrated in Jurkat cells. Tyrosine phosphorylation is expected at Tyr172, Tyr210, Tyr224, and Tyr282 by EGFR and at Tyr440 by InsR.
Catalogue Number654161
Brand Family Calbiochem®
SynonymsTubulin-α-1, Human, Recombinant
Application Data
References
ReferencesLennon, G., et al. 1996. Genomics 33, 151.
Cox, M.E. and Maness, P.F. 1993. J. Mol. Neurosci. 4, 63.
Matten, W.T., et al. 1990. J. Cell. Biol. 111, 1959.
Product Information
FormLiquid
FormulationIn PBS, 6 M Urea, 0.2% Protease Inhibitor Cocktail Set VII (Cat. No. 539138).
Biological Information
Purity≥90% by SDS-PAGE
Concentration Label Please refer to vial label for lot-specific concentration
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Irritant
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Global Trade Item Number
Catalogue Number GTIN
654161 0