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616379 Toxin A, Clostridium difficile - Calbiochem

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Enterotoxin

616379
  
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Overview

Replacement Information
Description
Overview

This product has been discontinued.



A high molecular weight glucosyltransferase that inhibits small GTPases, such as Rho, Rac, and Cdc42, by glucosylation of a threonine residue. This results in the shutdown of signal transduction cascades, leading to depolymerization of the cytoskeleton, gene transcription of a number of stress-activated protein kinases (SAPKs), and a decrease in the synthesis of phosphatidylinositol 4,5-bisphosphate. Unlike Exoenzyme C3 (Cat. No. 341208), this toxin does not require assistance to permeabilize cells.
Catalogue Number616379
Brand Family Calbiochem®
SynonymsEnterotoxin
References
ReferencesMackay, D.J. and Hall, A. 1998. J. Biol. Chem. 273, 20685.
Mahida, Y.R., et al. 1996. Gut 38, 337.
Wilkins, T.D. and Lyerly, D.M. 1996. Trends Microbiol. 4, 49.
Product Information
ATP CompetitiveN
FormSolid
FormulationLyophilized from 50 mM Tris, 50 mM NaCl, 0.1% trehalose, pH 7.5.
ReversibleN
Quality LevelMQ100
Applications
Biological Information
Biological activityBinds to fresh rabbit red blood cells in a hemagglutination assay. Cytotoxic as determined by a cell rounding assay using human foreskin cells
Primary TargetSmall GTPases, such as Rho, Rac, and Cdc42
PuritySingle band by SDS-PAGE
Physicochemical Information
Cell permeableN
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSKA8080200
Safety Information
R PhraseR: 26/27/28-36/37/38

Very toxic by inhalation, in contact with skin and if swallowed.
Irritating to eyes, respiratory system and skin.
S PhraseS: 26-36/37/39-45

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing, gloves and eye/face protection.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Highly Toxic
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, refrigerate (4°C). Stock solutions are stable for up to 1 week at 4°C. Toxin A activity was found to decrease to 1/10 after one week storage at 4°C following reconstitution.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
616379 0

Documentation

Toxin A, Clostridium difficile - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Toxin A, Clostridium difficile - Calbiochem Certificates of Analysis

TitleLot Number
616379

References

Reference overview
Mackay, D.J. and Hall, A. 1998. J. Biol. Chem. 273, 20685.
Mahida, Y.R., et al. 1996. Gut 38, 337.
Wilkins, T.D. and Lyerly, D.M. 1996. Trends Microbiol. 4, 49.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision19-May-2009 RFH
SynonymsEnterotoxin
DescriptionA high molecular weight glucosyltransferase that inhibits small GTPases, such as Rho, Rac, and Cdc42 by glucosylation of a threonine residue. This results in the shutdown of signal transduction cascades, leading to depolymerization of the cytoskeleton, gene transcription of a number of stress-activated protein kinases (SAPKs), and a decrease in the synthesis of phosphatidylinositol 4,5-bisphosphate. Unlike Exoenzyme C3 (Cat. No. 341208), this toxin does not require assistance to permeabilize cells.
FormSolid
FormulationLyophilized from 50 mM Tris, 50 mM NaCl, 0.1% trehalose, pH 7.5.
RTECSKA8080200
PuritySingle band by SDS-PAGE
Biological activityBinds to fresh rabbit red blood cells in a hemagglutination assay. Cytotoxic as determined by a cell rounding assay using human foreskin cells
SolubilityReconstitute with 50 µl of sterile, distilled H₂O.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, refrigerate (4°C). Stock solutions are stable for up to 1 week at 4°C. Toxin A activity was found to decrease to 1/10 after one week storage at 4°C following reconstitution.
Toxicity Highly Toxic
ReferencesMackay, D.J. and Hall, A. 1998. J. Biol. Chem. 273, 20685.
Mahida, Y.R., et al. 1996. Gut 38, 337.
Wilkins, T.D. and Lyerly, D.M. 1996. Trends Microbiol. 4, 49.