Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

GF102 TWEAK, human

Human TWEAK is a new secreted ligand in the tumor necrosis factor family that weakly induces apoptosis.

TWEAK, human MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Cell Culture UniProt Number: O43508 Entrez Gene Number: NM_003809.2
GF102
25 µg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Key ApplicationsSpeciesSourcePurity
CULTHuman E.coliGreater than 98% by SDS-PAGE and HPLC analyses
Description
Catalogue NumberGF102
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionTWEAK, human
OverviewHuman TWEAK is a new secreted ligand in the tumor necrosis factor family that weakly induces apoptosis. TWEAK, like many members of the TNF family, was able to induce interleukin-8 synthesis in a number of cell lines. GF102 is a 17.0 kDa protein, comprising the TNF-like extracellular domain of TWEAK.
References
Product Information
PresentationSterile filtered and then the protein was lyophilized from 10 mM NaP pH 7.5 + 0.1 M L-Arg.
Quality LevelMQ100
Applications
ApplicationHuman TWEAK is a new secreted ligand in the tumor necrosis factor family that weakly induces apoptosis.
Key Applications
  • Cell Culture
Biological Information
PurityGreater than 98% by SDS-PAGE and HPLC analyses
SourceE.coli
Specific ActivityBIOLOGICAL ACTIVITY: <br /><br />GF102 is fully biologically active when compared to standards. The ED50 as determined by the dose-dependent stimulation of IL-8 production by Human PBMC is less than 10ng/ml. <br /><br />GF102 weakly induces the death of HT29 cells when cultured in the presence of IFN-g. The ED50 for this effect is between 30-45 ng/ml.
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein is a ligand for the FN14/TWEAKR receptor. This cytokine has overlapping signaling functions with TNF, but displays a much wider tissue distribution. This cytokine can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. This cytokine is also found to promote proliferation and migration of endothelial cells, and thus acts as a regulator of angiogenesis.
Gene Symbol
  • TNFSF12
  • MGC129581
  • DR3LG
  • TWEAK
  • APO3L
  • MGC20669
UniProt Number
UniProt SummaryFUNCTION: SwissProt: O43508 # Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines.
SIZE: 249 amino acids; 27216 Da
SUBUNIT: Homotrimer (Potential). Interacts with the angiogenic factor AGGF1/VG5Q.
SUBCELLULAR LOCATION: Cell membrane; Single-pass type II membrane protein. & Tumor necrosis factor ligand superfamily member 12, secreted form: Secreted.
TISSUE SPECIFICITY: Highly expressed in adult heart, pancreas, skeletal muscle, brain, colon, small intestine, lung, ovary, prostate, spleen, lymph node, appendix and peripheral blood lymphocytes. Low expression in kidney, testis, liver, placenta, thymus and bone marrow. Also detected in fetal kidney, liver, lung and brain.
PTM: The soluble form derives from the membrane form by proteolytic processing.
SIMILARITY: SwissProt: O43508 ## Belongs to the tumor necrosis factor family.
Stem Cell Type
  • Mesenchymal Stem Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsThe lyophilized protein is stable for a few weeks at room temperature, but best stored at -20°C. Freeze/thaw cycles will result in significant loss of activity. After a quick spin, reconstitute in 10 mM NaP pH 7.5 to a concentration of 1 mg/ml. This solution can be diluted into other buffer solutions or stored at -20°C. Reconstituted TWEAK should be stored at -20°C or lyophilized in working aliquots.
Packaging Information
Material Size25 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
GF102 04053252511233

Documentation

TWEAK, human SDS

Title

Safety Data Sheet (SDS) 

TWEAK, human Certificates of Analysis

TitleLot Number
RECOMBINANT HUMAN TWEAK 2933274
RECOMBINANT HUMAN TWEAK 2898475
RECOMBINANT HUMAN TWEAK 3774189
RECOMBINANT HUMAN TWEAK 2898473
RECOMBINANT HUMAN TWEAK - 2388301 2388301
RECOMBINANT HUMAN TWEAK - 2051904 2051904
RECOMBINANT HUMAN TWEAK - 2080854 2080854
RECOMBINANT HUMAN TWEAK - 2089425 2089425
RECOMBINANT HUMAN TWEAK - 2231598 2231598
RECOMBINANT HUMAN TWEAK - 2565767 2565767
Show more

Data Sheet

Title
RECOMBINANT HUMAN TWEAK

Related Products & Applications

Product Families

Cytokines and Growth Factors

EMD Millipore offers a comprehensive range of highly purified bioactive cytokines and growth factors for use in human, mouse, and rat cell culture, stem cell differentiation and ELISA studies.
Learn More >>

Categories

Life Science Research > Proteins and Enzymes > Cytokines & Growth Factors
Life Science Research > Cell Culture and Systems > Cell Culture Media and Supplements > Cell Culture Supplements > Cytokines & Growth Factors