Stericap™ PLUS Universal Bottle-Top Filter Device
The Stericap™ PLUS Universal Bottle-Top Filter Device provides fast, large-volume filtration into any sized bottle.
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The Stericap™ PLUS Universal Bottle-Top Filter Device provides fast, large-volume filtration into any sized bottle. Less
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Overview
The Stericap™ PLUS Universal Bottle-Top Filter Device with Millipore Express® PLUS membrane provides fast vacuum filtration and low protein binding without the need for a prefilter in most cases. It filters quickly, without clogging, even viscous solutions typical of serum-containing media. This non-cytotoxic, non-pyrogenic device can be used to fill any vacuum-rated bottle or other container with an inner neck diameter of 20 to 67 mm. This allows maximum versatility, capacity, speed, and flowthrough efficiency when you need to filter larger volumes into containers with openings of various sizes.
Features & Benefits
- Millipore Express® PLUS membrane for fastest flow rates and low protein binding
- Fits any vacuum-rated bottle from 20 to 67 mm in diameter for maximum flexibility
- High volume capacity for larger and less frequent preparation steps
- Easy flow control button so the filter can be easily transferred from bottle to bottle without turning off the vacuum each time
- Free of cytotoxic and pyrogenic contaminants for robust cell culture and sensitive downstream applications
High Flow and High Throughput
Reconstituted DMEM with 10% fetal bovine serum was filtered through 3 Stericap PLUS devices and competitive bottle-top devices (2 each). The data show the average flow times.
Applications
Sterilization of Aqueous Solutions, Media Preparation, Buffer Preparation
Product Performance
Specifications
| Materials | |
| Housing | Styrene/Polypropylene/Polyethylene |
| Membrane (Millipore Express® PLUS) | Polyethersulfone (PES) |
| Pore Size | 0.22 µm |
| Connections | |
| Inlet Connection | Hose barb |
| Outlet Connection | Universal bottle top |
| Vent | Needle valve with female Luer-Lok® |
| Vacuum Port | Hose barb |
| Filtration Area | 40 cm² |
| Maximum Operating Vacuum | 635 mm Hg (25" Hg) |
| Sterilization | Gamma irradiation |
Ordering Information
Documentation
FAQ
| Question | Answer |
|---|---|
| Is the Stericap PLUS autoclavable? | No, Stericap PLUS is a single-use device. The highest temperature that the product can be operated at is 45C (113F ). The tubing set which is provided is autoclave compatible for at least 10 cycles. After the first autoclave cycle, the tubing may lose some of its transparency and the tube weight will appear lighter in color, but this is purely cosmetic and does not affect its function nor lead to additional extractables. |
| How can I clean the Stericap PLUS tubeset between uses? Is it autoclaveable? | The tubeset provided with the Stericap PLUS can be cleaned with detergent and water. Be certain to thoroughly rinse the tubeset if detergent is used. The tubing set which is provided is autoclave compatible for at least 10 cycles at 121oC for 15 minutes. After the first autoclave cycle, the tubing may lose some of its transparency and the tube weight will appear lighter in color, but this is purely cosmetic and does not affect its function nor lead to additional extractables. |
| How long can I be certain that the Stericap PLUS is sterile after I purchase it? | Millipore guarantees sterility of the Stericap PLUS for 3 years from date of manufacture. |
| What volume can I filter through my Stericap PLUS? | The Stericap PLUS is recommended for volumes of 5-10 L. Actual throughput depends on the specific nature of the sample being filtered. Competitive performance data were generated using DMEM with 10% Serum, and Stericap PLUS was shown to filter at least 10 L. |
| What flow rates can I expect from my Stericap PLUS? | Stericap PLUS has a QC release specified clean water flow time of <52 seconds for 500 mL (minimum average flow rate of 577 mL/min) at a vacuum level of 25” Hg. Filtration of more viscous or particulate laden solutions will result in lower flow rates, and instantaneous flow rates that reduce over time. For example in competitive performance testing, average flow rate over 10 minutes for DMEM with 10% serum was 550 mL/min, while over 20 minutes it was 375 mL/minute, and over 60 minutes, it was 167 mL/min. |
| What is the hold up volume in the Stericap PLUS unit? | Stericap PLUS holdup volume is <20 mL. |
| Can I bubble point my Stericap PLUS unit? | Since it is a vacuum driven device, Stericap PLUS is not configured to allow bubble point testing. The housing leak integrity test on the housing is performed at 15 psig, which is significantly lower than the water bubble point of the membrane. |
| Some liquid I was filtering was aspirated into my vacuum line. How do I prevent that from happening? | You should stop filtering before the liquid level reaches the top of the bottle to prevent liquid from entering the vacuum line. To protect you vacuum source from serious damage which can be caused when liquid accidentally enters the vacuum tubing, your setup should consist of both a trap followed by a Millex FG 50 (SLFG05010) hydrophobic vent filter. |
| Can I reuse my Stericap PLUS? | Stericap PLUS is a disposable filtration unit intended for single use only. |
| Which receiver bottles can I use with my Stericap PLUS? | Stericap PLUS is a universal bottle top filtration device. It is compatible with bottles which have neck inner diameter sizes as small as 20mm up to wide mouth bottles with inner neck diameter of 67mm. Any glass or plastic receiver bottle used should be rated for use with vacuum. Millipore offers disposable, plastic, vacuum rated 1 Liter receiver bottles (SC00B10RE). |
Related Products & Applications
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| Dialysis and Filtration |
Related Products By: Brand Facete
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Categories
| Life Science Research > Cell Culture and Systems > Sterile Filtration Devices |
| Materials | |
| Housing | Styrene/Polypropylene/Polyethylene |
| Membrane (Millipore Express® PLUS) | Polyethersulfone (PES) |
| Pore Size | 0.22 µm |
| Connections | |
| Inlet Connection | Hose barb |
| Outlet Connection | Universal bottle top |
| Vent | Needle valve with female Luer-Lok® |
| Vacuum Port | Hose barb |
| Filtration Area | 40 cm² |
| Maximum Operating Vacuum | 635 mm Hg (25" Hg) |
| Sterilization | Gamma irradiation |