Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

566660 Smoothened Agonist, SAG - CAS 364590-63-6 - Calbiochem

A cell-permeable Smoothened Agonist, SAG, CAS 364590-63-6, modulates the coupling of Smo with its downstream effector by interacting with the Smo heptahelical domain (KD = 59 nM).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: N-Methyl-Nʹ-(3-pyridinylbenzyl)-Nʹ-(3-chlorobenzo[b]thiophene-2-carbonyl)-1,4-diaminocyclohexane, SAG1.3

566660
Purchase on Sigma-Aldrich

Overview

Key Spec Table

CAS #Empirical Formula
364590-63-6C₂₈H₂₈ClN₃OS • 2HCl • 2H₂O

Products

Catalogue NumberPackaging Qty/Pack
566660-1MG Glass bottle 1 mg
Add To Favorites
566660-5MG Glass bottle 5 mg
Add To Favorites
Description
OverviewA cell-permeable benzothiophene compound that modulates the coupling of Smo with its downstream effector by interacting with the Smo heptahelical domain (KD = 59 nM). Shown to induce Smo internalization. Shown to induce Hedgehog pathway activation (EC50 = ∼ 3 nM in NIH 3T3-derived Shh-LIGHT2 cells) and counteracts Cyclopamine-KAAD (Cat. No. 239804) inhibition of Smo. Reported to act as an activator at low concentrations and as an inhibitor at very high concentrations. A 10 mM (500 µg/86 µl) solution of Smoothened Agonist, SAG (Cat. No. 566661) in H2O is also available.
Catalogue Number566660
Brand Family Calbiochem®
SynonymsN-Methyl-Nʹ-(3-pyridinylbenzyl)-Nʹ-(3-chlorobenzo[b]thiophene-2-carbonyl)-1,4-diaminocyclohexane, SAG1.3
References
ReferencesWang, J., et al. 2010. Proc. Natl. Acad. Sci. USA. 107, 9323.
Meloni, A.R., et al. 2006. Mol. Cell. Biol. 26, 7750.
Chen, W., et al. 2004. Science 306, 2257.
Chen, J.K., et al. 2002. Proc. Natl. Acad. Sci. USA 99, 14071.
Frank-Kamenetsky, M., et al. 2002. J. Biol. 1, 10.
Product Information
CAS number364590-63-6
FormLight yellow solid
Hill FormulaC₂₈H₂₈ClN₃OS • 2HCl • 2H₂O
Chemical formulaC₂₈H₂₈ClN₃OS • 2HCl • 2H₂O
Hygroscopic Hygroscopic
Structure formula ImageStructure formula Image
Quality LevelMQ100
Biological Information
Purity≥98% by HPLC
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Light Protect from light
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Global Trade Item Number
Catalogue Number GTIN
566660-1MG 07790788051570
566660-5MG 04055977191578