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03-0187-00 SMC™ Human MCP-1 High Sensitivity Immunoassay Kit

The sensitivity of the Single Molecule Counting (SMC™) technology gives the SMC™ Human MCP-1 High Sensitivity Immunoassay Kit unparalleled performance in the quantification of MCP-1 in human serum and plasma.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Single Molecule Counting (SMC®) Research Category: Inflammation & Immunology Research Sub-Category: Inflammatory Disease, Immune Response, Autoimmune disease
03-0187-00
96 well assay  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Key Applications
Single Molecule Counting (SMC®)
Description
Catalogue Number03-0187-00
Trade Name
  • SMC®
DescriptionSMC™ Human MCP-1 High Sensitivity Immunoassay Kit
OverviewThe SMC™ Human MCP-1 High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The Erenna® and SMCxPRO™ immunoassay systems detect analytes using Single Molecule Counting (SMC™) technology.
Background InformationMonocyte Chemoattractant Protein-1 (MCP-1) also referred to as CCL2 is a chemokine critical in regulating the migration and infiltration of monocyte and macrophages from the bloodstream in response to inflammation. It has been shown to play a role in the pathogenesis of diseases like psoriasis, atherosclerosis and rheumatoid arthritis characterized by monocyte infiltration. As well, MCP-1 plays a role in central nervous system diseases like Alzheimer’s disease, traumatic brain injury and epilepsy where expression in glial cells has been shown to be increased.
References
Product Information
Components
  • Assay Buffer
  • MCP-1 Coated Beads
  • Standard Diluent
  • MCP-1 Detection Antibody
  • MCP-1 Standard
  • 10X Wash Buffer
  • Buffer D
  • Elution Buffer B
Precision, %
  • The assay variations of SMC™ Human MCP-1 Immunoassay kit were studied using five normal plasma samples run in triplicate by 3 different operators on 3 different days.
  • Intra-assay CV% average : 5%
  • Inter-assay CV% average : 4%
Applications
ApplicationThe sensitivity of the Single Molecule Counting (SMC™) technology gives the SMC™ Human MCP-1 High Sensitivity Immunoassay Kit unparalleled performance in the quantification of MCP-1 in human serum and plasma.
Key Applications
  • Single Molecule Counting (SMC®)
Application NotesThis new streamline kit has the following features including:
• Improved options for sample prep and incubation
• Lyophilization of the standard provides the kit at one storage condition
• Removal of a plate transfer step
• Increased reagent volumes to accommodate automation
• Validated to run on both SMCxPRO™ and Erenna®
Biological Information
Species Reactivity NoteSpecificity to the following species analytes were tested with the following results:
Rat – Not cross-reactive
Mouse – Not cross-reactive
Canine – Not cross-reactive
Cross ReactivityCross-reactivity to the following analytes were tested with the following results:
MCP-2 – Not cross-reactive
MCP-3 – Not cross-reactive
Accuracy
  • Minimum of 5 serum samples spiked with average recovery of 104% with a range of 92-115%
  • Minimum of 5 plasma samples spiked with average recovery of 101% with a range of 80-123%
Physicochemical Information
Sensitivity
  • Lower Limit of Quantification: 0.15 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size96 well assay
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
03-0187-00 04061841929932