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03-0203-00 SMC™ Human GFAP High Sensitivity Immunoassay Kit

The sensitivity of the Single Molecule Counting (SMC™) technology gives the SMC™ Human GFAP High Sensitivity Immunoassay Kit unparalleled performance in the quantification of GFAP in human serum, plasma, and CSF.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Single Molecule Counting (SMC®) Research Category: Neuroscience, neurological Research Sub-Category: Alzheimer's Disease, Aging, Nervous System, Neurodegenerative Diseases, Neurofilament & Neuron Metabolism, Neuronal & Glial Markers, Neuroregenerative Medicine, Neuroscience
03-0203-00
96 well assay  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Key Applications
Single Molecule Counting (SMC®)
Description
Catalogue Number03-0203-00
Trade Name
  • SMC®
DescriptionSMC™ Human GFAP High Sensitivity Immunoassay Kit
OverviewThe SMC™ Human GFAP High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The Erenna® and SMCxPRO™ immunoassay systems detect analytes using Single Molecule Counting (SMC™) technology.
Background InformationGlial fibrillary acidic protein (GFAP) is a type III intermediate filament protein produced notably by astrocytes during CNS development, and functions in astrocyte regeneration, plasticity, and also contributes to function of the blood brain barrier. GFAP is a potential biomarker in a number of neurodegenerative diseases as well as in traumatic brain injury. There is much interest today in measuring GFAP levels in low abundance, because not only does it make it possible to evaluate this protein as a blood biomarker, but also enables researchers to study the downregulation of GFAP in therapeutic research. The new SMC™ Human GFAP High Sensitivity Immunoassay Kit enables accurate measurement of this protein in human serum, plasma, and cerebrospinal fluid, harnessing the power of the SMCxPRO™ ultrasensitive immunoassay system.
References
Product Information
Components
  • Assay Buffer
  • GFAP Coated Beads
  • Standard Diluent
  • GFAP Detection Antibody
  • GFAP Standard
  • 10X Wash Buffer
  • Buffer D
  • Elution Buffer B
Precision, %
  • The assay variations of SMC™ Human GFAP Immunoassay kit were studied
    using five normal plasma samples run in triplicate by 3 different operators on 3 different days.
  • Intra-assay CV% average : ≤ 5%
  • Inter-assay CV% average : ≤ 6%
Quality LevelMQ200
Applications
ApplicationThe sensitivity of the Single Molecule Counting (SMC™) technology gives the SMC™ Human GFAP High Sensitivity Immunoassay Kit unparalleled performance in the quantification of GFAP in human serum, plasma, and CSF.
Key Applications
  • Single Molecule Counting (SMC®)
Application NotesThis new streamline kit has the following features:
• Improved options for sample prep and incubation
• Lyophilization protein standard for uniform shipping and storage condition
• Increased reagent volumes to accommodate automation
• Able to achieve assay specifications on both SMCxPRO™ and Erenna®.
Biological Information
Species Reactivity NoteSpecificity to the following species samples were tested with the follow results:
Non Human Primate – 100%
Mouse – 100%
Rat – 100%
Canine – 100%
Feline – 100%
Cross ReactivityThis assay was tested for cross-reactivity with Ventimin, NFL, UCHL1 and total Tau. No cross-reactivity was detected with any of these analytes.
Accuracy
  • Minimum of 4 serum samples spiked with average recovery of 118% with a range of 113-123%
  • Minimum of 4 plasma samples spiked with average recovery of 115% with a range of 110-119%
  • Minimum of 5 CSF samples spiked with average recovery of 101% with a range of 95-106%
Physicochemical Information
Sensitivity
  • Lower Limit of Quantification: 0.23 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size96 well assay
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
03-0203-00 04065266870381