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14-331 SGK1 (δ1-59, S422D) Protein, active, 6 µg

Active, N-truncated (missing N-terminal 60 amino acids), His-tagged, human SGK1 fusion protein, containing a S422D mutation, for use in Kinase Assays.

SGK1 (δ1-59, S422D) Protein, active, 6 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-331
6 µg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number14-331
Brand Family Upstate
Trade Name
  • Upstate
DescriptionSGK1 (δ1-59, S422D) Protein, active, 6 µg
OverviewN-truncated (missing Nterminal 60 amino acids), His-tagged, human SGK1 fusion protein, containing a S422D mutation
References
Product Information
Quality LevelMQ100
Applications
ApplicationActive, N-truncated (missing N-terminal 60 amino acids), His-tagged, human SGK1 fusion protein, containing a S422D mutation, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
Sourceexpressed in Sf21 cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a serine/threonine protein kinase that is highly similar to the rat serum-and glucocorticoid-induced protein kinase (SGK). This gene was identified in a screen of hepatocellular genes regulated in response to cellular hydration or swelling. Cellular hydration is a catabolic signal, stimulating glycogenolysis and proteolysis, and inhibiting protein and glycogen synthesis. This kinase has been shown to be important in activating certain potassium, sodium, and chloride channels. Expression of this gene in hepatocytes is stimulated by transforming growth factor-beta (TGF-beta) which participates in the pathophysiology of diabetic complications. Since both TGF-beta expression and SGK expression are elevated in diabetic nephropathy, this suggests an involvement of SGK in the development of this condition.
Gene Symbol
  • SGK
  • SGK1
Protein TargetSGK1
Purification MethodNi-NTA agarose
Target Sub-FamilyAGC
UniProt Number
UniProt SummaryFUNCTION: SwissProt: O00141 # Protein kinase that plays an important role in cellular stress response. Activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. Sustained high levels and activity may contribute to conditions such as hypertension and diabetic nephropathy. Mediates cell survival signals, phosphorylates and negatively regulates pro-apoptotic FOXO3A. Phosphorylates NEDD4L, which leads to its inactivation and to the subsequent activation of various channels and transporters such as ENaC, Kv1.3, or EAAT1.
SIZE: 431 amino acids; 48942 Da
SUBUNIT: Interacts with NEDD4 and NEDD4L.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Endoplasmic reticulum. Note=Nuclear, upon phosphorylation.
TISSUE SPECIFICITY: Expressed in most tissues with highest levels in the pancreas, followed by placenta, kidney and lung.
PTM: Regulated by phosphorylation. Phosphoinositide 3-kinase (PI3- kinase) pathway promotes phosphorylation at Ser-422 which in turn increases the phosphorylation of Thr-256 by PDPK1. & Ubiquitinated by NEDD4L; which promotes proteasomal degradation. Ubiquitinated by SYVN1 at the endoplasmic reticulum; which promotes rapid proteasomal degradation and maintains a high turnover rate in resting cells.
SIMILARITY: SwissProt: O00141 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 protein kinase domain.
Molecular Weight48kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by Kinase assay
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions1 year at -70°C
Packaging Information
Material Size6 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
14-331 04053252328633

Documentation

SGK1 (δ1-59, S422D) Protein, active, 6 µg SDS

Title

Safety Data Sheet (SDS) 

SGK1 (δ1-59, S422D) Protein, active, 6 µg Certificates of Analysis

TitleLot Number
SGK1 (S422D), active - 1797670-A 1797670-A
SGK1 (S422D), active - 1797670-B 1797670-B
SGK1 (S422D), active - 28638U-G 28638U-G

References

Reference overviewApplicationPub Med ID
Activation of NHE3 by dexamethasone requires phosphorylation of NHE3 at Ser663 by SGK1
Wang, Dongsheng, et al
Am J Physiol, Cell Physiol, 289:C802-10 (2005)  2005

15888551 15888551
Cell surface expression of the ROMK (Kir 1.1) channel is regulated by the aldosterone-induced kinase, SGK-1, and protein kinase A
Yoo, D., et al
J Biol Chem, 278:23066-75 (2003)  2003

Kinase Assay12684516 12684516
Characterization of the structure and regulation of two novel isoforms of serum- and glucocorticoid-induced protein kinase
Kobayashi, T., et al
Biochem J, 344 Pt 1:189-97 (1999)  1999

Protein Kinase/Phosphatase Assays10548550 10548550