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70920 Rosetta™(DE3)pLacI Competent Cells - Novagen

Rosetta strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. Contains the pLacI plasmid producing extra Lac repressor.

Rosetta™(DE3)pLacI Competent Cells - Novagen MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
70920
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
70920-3 Glass bottle 0.4 ml
70920-4 Plastic ampoule 1 ml
Description
OverviewExpression host strains for pETBlue™ and pTriEx™ vectors are lDE3 lysogens that also carry the compatible pLacI plasmid, encoding the lac repressor. The pLacI plasmid confers chloramphenicol resistance and provides enough lac repressor to inhibit transcription from the T7lac promoter in the absence of inducer. The NovaBlue host strain is recommended for initial cloning with pETBlue and pTriEx vectors due to its superior transformation efficiency and high yields of high-quality plasmid DNA.

Note that these strains are not recommended for use with pET, pACYCDuet™, pCDF, pRSF, pCOLADuet™ or pETcoco™ vectors.

.Rosetta™ host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. These strains supply tRNAs for 7 rare codones (AGA, AGG, AUA, CUA, GGA, CCC, and CGG) on a compatible chloramphenicol-resistant plasmid. The tRNA genes are driven by their native promoters. In Rosetta(DE3)pLacI, the rare tRNA genes are present on the same plasmid that carries the lac repressor gene.

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
Genotype: F– ompT hsdSB (rB– mB–) gal dcm (DE3) pLacIRARE (CamR)


This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.




This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
Catalogue Number70920
Brand Family Novagen®
References
Product Information
Components
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage ≤ -70°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
70920-3 07790788052881
70920-4 04055977272710

Documentation

Rosetta™(DE3)pLacI Competent Cells - Novagen SDS

Title

Safety Data Sheet (SDS) 

Rosetta™(DE3)pLacI Competent Cells - Novagen Certificates of Analysis

TitleLot Number
70920

Citations

Title
  • Jason Cellitti, et al. (2007) Exploring subdomain cooperativity in T4 lysozyme I: Structural and energetic studies of a circular permutant and protein fragment. Protein Science 16, 842-851.
  • Alina Castell, et al. (2005) Rv0216, a conserved hypothetical protein from Mycobacterium tuberculosis that is essential for bacterial survival during infection, has a double hotdog fold. Protein Science 14, 1850-1862.
  • Sasha F. Levy, et al. (2005) Three-and four-repeat tau regulate the dynamic instability of two distinct microtubule subpopulations in qualitatively different manners: implications for neurodegeneration. Journal of Biological Chemistry 280, 13520-13528.
  • Yasodha Natkunam, et al. (2005) Expression of the human germinal center-associated lymphoma (HGAL) protein, a new marker of germinal center B-cell derivation. Blood 105, 3979-3986.
  • Satoko Yoshida and Martin Parniske. (2005) Regulation of plant symbiosis receptor kinase through serine and threonine phosphorylation. Journal of Biological Chemistry 280, 9203-9209.
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    User Protocols

    Title
    TB009 Competent Cells
    TB053 Academic and Non-profit Laboratory Assurance Letter
    TB249 pETBlue™ System Manual
    TB250 pTriEx™ System Manual