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HTS114RTA Ready-to-Assay™ PAC1 (long isoform) VIP/PACAP Receptor Frozen Cells

Human PAC1 long isoform / PACAP GPCR frozen cells for Calcium Flux FLIPR Assays.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Host Cell: Chem-1 Entrez Gene Number: NM_001118
HTS114RTA
1 kit  
Purchase on Sigma-Aldrich

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Overview

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Key Spec Table

SpeciesHost CellsProtein Target
Human Chem-1PAC1 long isoform / PACAP
Description
Catalogue NumberHTS114RTA
Trade Name
  • Ready-to-Assay
DescriptionReady-to-Assay™ PAC1 (long isoform) VIP/PACAP Receptor Frozen Cells
Background InformationMillipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

PACAP (pituitary adenylyl cyclase-activating peptide) is a peptide that exists in 2 forms, 27 or 38 amino acids, and is related to vasoactive intestinal peptide (VIP). Three related class B GPCRs, PAC1, VPAC1 and VPAC2, bind to PACAP; however, VPAC1 and VPAC2 have a much higher affinity for VIP than does PAC1 (Vaudry et al., 2000). Several splice variants of PAC1 result in proteins that differ at the N-terminus and third intracellular loop; these variants differ in their affinities for PACAP and abilities to activate Gq and Gs. High expression of PAC1 is observed in the CNS and the adrenal medulla. Studies with PAC1-null mice indicate that PAC1 plays important roles in regulation of circadian rhythms, neutrophil migration, and pulmonary vascular tone (Hannibal et al., 2001; Martinez et al., 2005; Otto et al., 2004). Millipore’s cloned human PAC1-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant PAC1 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at PAC1 Receptor.
Product Information
Components
  • Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
  • Fifty (50) mL of Media Component.
Detection methodFluorescent
Quality LevelMQ100
Applications
ApplicationHuman PAC1 long isoform / PACAP GPCR frozen cells for Calcium Flux FLIPR Assays.
Key Applications
  • Calcium Flux Assays
Biological Information
Entrez Gene Number
GPCR ClassB
Host CellsChem-1
Protein TargetPAC1 long isoform / PACAP
Target Sub-FamilyVIP/PACAP
Cell Line Type
  • GPCR Cell Lines
Product Usage Statements
Usage Statement
  • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Packaging Information
Material Size1 kit
Global Trade Item Number
Catalogue Number GTIN
HTS114RTA 04053252481567