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539791 ProteoExtract® Subcellular Proteome Extraction Kit, Mini

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: S-PEK Kit, Mini

539791
  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Overview

This product has been discontinued.



The ProteoExtract® Subcellular Proteome Extraction Kit, Mini is a tool for the differential extraction of proteins from mammalian cells according to their subcellular localization. The sequential extraction steps yield proteins in their native state, making it highly suited for demanding proteomics applications. Sample size: 3-5x106 or 25-50 mg tissue.
Catalogue Number539791
Brand Family Calbiochem®
SynonymsS-PEK Kit, Mini
Materials Required but Not Delivered• Platform mixer (e.g. IKA Vibramax; for extracting adherent tissue culture cells)
• Cell culture equipment, medium, and other reagents required for cell culture
• Pipettors and tips for 10 µl, 200 µl, and 1 ml
• Cooled centrifuge and rotor for 50 ml tube size
• Cooled microcentrifuge and rotor up to 10,000 x g for 2 ml tube size
• Thermo mixer or rolling facility
References
ReferencesYuan, X., et al. 2002. Electrophoresis 23, 1185.
Butcher, B.A., et al. 2001. J. Immunol. 167, 2193;
Ott, V., et al. 2001. Pharmacogenomics J. 1, 142.
Allen, L., et al. 2000. Nature 405, 819.
Rabilloud, T. 2000. Anal. Chem. 72, 48A.
Mejdoubi, M., et al. 1999. Biochem. Biophys. Res. Commun. 254, 93.
Reymond, et al. 1997. Electrophoresis 18, 2842.
Laemmli, U. K. 1970. Nature 227, 680.
Lowry, O.H., et al. 1951. J. Biol. Chem. 193, 265.
Product Information
Form10 Extractions
Kit containsWash buffer, Extraction Buffer I, Extraction Buffer II, Extraction Buffer III, Extraction Buffer IV, Protease Inhibitor Cocktail (Cat. No. 539134), Benzonase® Nuclease (Cat. No. 71206), and a user protocol.
Applications
Biological Information
Sample TypeMammalian tissues and cultured cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Intended useThe Calbiochem® ProteoExtract® Subcellular Proteome Extraction Kit Mini (S-PEK) is intended for the differential extraction of proteins from mammalian cells according to their subcellular localization. The procedure yields proteins in their native state making it highly suited for demanding proteomics applications including enzyme activity assays (e.g. reporter gene assays) and subcellular redistribution assays to monitor protein shuttling (e.g. signaling proteins).
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage +2°C to +8°C
Storage ConditionsUpon arrival store the components of the kit under the following conditions:

• Wash Buffer: Store at 4°C.
• Extraction Buffers I-III: Store at 4°C for up to 6 months. For long-term storage, freeze the buffers in convenient aliquots at -20°C.
• Extraction Buffer IV: Store at room temperature.
• Protease Inhibitor Cocktail: The cocktail is supplied in DMSO and can be stored at 4°C for up to 6 months. For prolonged storage, freeze the cocktail in convenient aliquots at -20°C.
• Benzonase® Nuclease: Store at 4°C.
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Kit containsWash buffer, Extraction Buffer I, Extraction Buffer II, Extraction Buffer III, Extraction Buffer IV, Protease Inhibitor Cocktail (Cat. No. 539134), Benzonase® Nuclease (Cat. No. 71206), and a user protocol.
Specifications
Global Trade Item Number
Catalogue Number GTIN
539791 0

Documentation

ProteoExtract® Subcellular Proteome Extraction Kit, Mini SDS

Title

Safety Data Sheet (SDS) 

ProteoExtract® Subcellular Proteome Extraction Kit, Mini Certificates of Analysis

TitleLot Number
539791

References

Reference overview
Yuan, X., et al. 2002. Electrophoresis 23, 1185.
Butcher, B.A., et al. 2001. J. Immunol. 167, 2193;
Ott, V., et al. 2001. Pharmacogenomics J. 1, 142.
Allen, L., et al. 2000. Nature 405, 819.
Rabilloud, T. 2000. Anal. Chem. 72, 48A.
Mejdoubi, M., et al. 1999. Biochem. Biophys. Res. Commun. 254, 93.
Reymond, et al. 1997. Electrophoresis 18, 2842.
Laemmli, U. K. 1970. Nature 227, 680.
Lowry, O.H., et al. 1951. J. Biol. Chem. 193, 265.

Brochure

Title
Kit SourceBook - 2nd Edition EURO