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71771 ProteoExtract® S-PEK Antibody Control Kit

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Overview

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Description
Overview

This product has been discontinued.



The ProteoExtract® S-PEK™ Antibody Control Kit is a set of four monoclonal antibodies that recognize proteins specific for each of the four sub-cellular fractions obtained with the ProteoExtract Subcellular Proteome Extraction Kit (S-PEK). These antibodies can be used in Western blot procedures to confirm proper sub-fractionation of cellular proteomes. The kit provides 15 mg/vial each of the following antibodies: Anti-HSP 90, Anti-Vimentin, Anti-Calnexin, Anti-PARP. HSP90 protein is a marker for the cytosolic fraction, Calnexin is a trans-membrane protein, PARP is a nuclear protein, and Vimentin is an intermediate filament protein found in the cytoskeleton. Due to the specificity of Anti-HSP 90 and Anti-Vimentin antibodies, we recommend to use this kit with cellular fractions obtained from human-derived cell lines and tissues.
Catalogue Number71771
Brand Family Novagen®
Application Data
Subcellular fractions from suspension SAOS-2 cells were prepared according to the S-PEK protocol and analyzed by Western blot. For each blot, 2 µg of total protein from S-PEK fractions F1, F2, F3, and F4 was run on an 8-12% SDS-PAGE gel and transferred to a nitrocellulose membrane. The membranes were blocked with PBST containing 5% (w/v) non-fat dry milk for 30 min at room temperature and then incubated in a solution containing the primary antibody for 1 h at room temperature. After 3 washes with PBST, blots were incubated with a goat anti-mouse HRP-conjugated secondary antibody at a 1:10,000 dilution for 30 min at room temperature. Blots were washed three times with PBST and developed using a chemiluminescnet substrate. For all blots, lane 1: molecular weight markers; lane 2: S-PEK fraction F1 (cytoplasmic protein); lane 3: S-PEK fraction F2 (membrane/organelle proteins); lane 4: S-PEK fraction F3 (nuclear proteins); lane 5: S-PEK fraction F4 (cytoskeletal proteins).
References
Product Information
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Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
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Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
71771 0

Documentation

ProteoExtract® S-PEK Antibody Control Kit Certificates of Analysis

TitleLot Number
71771