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540020 Pluripotin

Pluripotin MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: N-(3-(7-(1,3-dimethyl-1H-pyrazol-5-ylamino)-1-methyl-2-oxo-1,2-dihydropyrimido[4,5-d]pyrimidin-3(4H)-yl)-4-methylphenyl)-3-(trifluoromethyl)benzamide, ERK Inhibitor VII, Ras GTPase-Activating Protein Inhibitor II, SC1

540020
  
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Overview

Replacement Information
Description
Overview

This product has been discontinued.



A cell-permeable dihydropyrimido-pyrimidine compound that effectively upregulates GTP-bound active Ras level and inhibits LIF- (leukemia inhibitory factor) induced Erk1/2 phosphorylation in OG2-mES (murine Embryonic Stem) cells (1 µM) via direct interaction with Erk and RasGAP (KD = 212 and 98 nM in binding RasGAP 729-1042 catalytic domain and Erk1, respectively), without affecting cellular STAT3 or GSK-3β/Wnt signaling. Pluripotin/SC1 is shown to greatly complement and enhance the efficiency of LIF in deriving ESCs from blastocysts of several murine strains and effectively maintain the pluripotent state of the ESCs even after 11 passages in cultures. SC1-expanded ESCs effectively form EBs (embryonic bodies) in vitro upon SC1 and LIF withdrawal and are capable of generating chimeric offsprings from surrogate mice when injected into blastocysts from different strains in vivo.
Catalogue Number540020
Brand Family Calbiochem®
SynonymsN-(3-(7-(1,3-dimethyl-1H-pyrazol-5-ylamino)-1-methyl-2-oxo-1,2-dihydropyrimido[4,5-d]pyrimidin-3(4H)-yl)-4-methylphenyl)-3-(trifluoromethyl)benzamide, ERK Inhibitor VII, Ras GTPase-Activating Protein Inhibitor II, SC1
References
ReferencesYang, W., et al. 2009. Stem Cells 27, 383.
Chen, S., et al. 2006. Proc. Natl. Acad. Sci. USA 103, 17266.
Product Information
FormYellow solid
Hill FormulaC₂₇H₂₅F₃N₈O₂
Chemical formulaC₂₇H₂₅F₃N₈O₂
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Purity≥97% by HPLC
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
540020 0

Documentation

Pluripotin SDS

Title

Safety Data Sheet (SDS) 

Pluripotin Certificates of Analysis

TitleLot Number
540020

References

Reference overview
Yang, W., et al. 2009. Stem Cells 27, 383.
Chen, S., et al. 2006. Proc. Natl. Acad. Sci. USA 103, 17266.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision10=February-2011 RFH
SynonymsN-(3-(7-(1,3-dimethyl-1H-pyrazol-5-ylamino)-1-methyl-2-oxo-1,2-dihydropyrimido[4,5-d]pyrimidin-3(4H)-yl)-4-methylphenyl)-3-(trifluoromethyl)benzamide, ERK Inhibitor VII, Ras GTPase-Activating Protein Inhibitor II, SC1
DescriptionA cell-permeable dihydropyrimido-pyrimidine compound that effectively upregulates GTP-bound active Ras level and inhibits LIF- (leukemia inhibitory factor) induced Erk1/2 phosphorylation in OG2-mES (murine Embryonic Stem) cells (1 µM) via direct interaction with Erk and RasGAP (KD = 212 and 98 nM in binding RasGAP 729-1042 catalytic domain and Erk1, respectively), without affecting cellular STAT3 or GSK-3β/Wnt signaling. Pluripotin/SC1 is shown to greatly complement and enhance the efficiency of LIF in deriving ESCs from blastocysts of several murine strains and effectively maintain the pluripotent state of the ESCs even after 11 passages in cultures. SC1-expanded ESCs effectively form EBs (embryonic bodies) in vitro upon SC1 and LIF withdrawal and are capable of generating chimeric offsprings from surrogate mice when injected into blastocysts from different strains in vivo.
FormYellow solid
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₂₇H₂₅F₃N₈O₂
Structure formulaStructure formula
Purity≥97% by HPLC
SolubilityDMSO (50 mg/ml)
Storage Protect from light
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesYang, W., et al. 2009. Stem Cells 27, 383.
Chen, S., et al. 2006. Proc. Natl. Acad. Sci. USA 103, 17266.