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525189 Phospholipase C-γ-2, His•Tag®, Human, Recombinant, S. frugiperda

525189
  
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Overview

Description
Overview

This product has been discontinued.



Full-length, recombinant, human phospholipase C γ-2 fused at the N-terminus to His•Tag® and S•Tag™ sequences and expressed in S. frugiperda insect cells. This preparation is qualified for use as a substrate for protein tyrosine kinases in in vitro assays. Intrinsic enzyme activity has not been tested. Production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes, which are crucial enzymes in transmembrane signaling. PLC-γ-2 is phosphorylated on tyrosine residues following ligand-induced activation of a variety of growth factor receptors and immune system receptors, resulting in increased phospholipase activity. The full-length proteins contains the following domains: C2 domain (aa 1059-1152); PH domain (aa 20-131); PI-PLC X-box catalytic domain (aa 312-456); PI-PLC Y-box catalytic domain (aa 930-1044); two SH2 domains (aa 532-635, aa 646-735); and a SH3 domain (aa 769-829). It has been reported to be phosphorylated at Tyr753 and Tyr759 (Btk, Src, Fyn, Lck), and Tyr1217 and Tyr1245 by Bcr-Abl.

Catalogue Number525189
Brand Family Calbiochem®
SynonymsPhospholipase C-γ-2, Human, Recombinant
Application Data
References
ReferencesRodriguez, R., et al. 2001. J. Biol. Chem. 276, 47982.
Lennon, G., et al. 1996. Genomics 33, 151.
Product Information
FormLiquid
FormulationIn PBS.
Quality LevelMQ100
Biological Information
Purity≥90% by SDS-PAGE
Concentration Label Please refer to vial label for lot-specific concentration
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Irritant
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Global Trade Item Number
Catalogue Number GTIN
525189 0