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553009 PhosphoDetect™ Anti-c-Raf (pTyr³⁴⁰, Tyr³⁴¹) Rabbit pAb

This PhosphoDetect™ Anti-c-Raf (pTyr³⁴⁰, Tyr³⁴¹) Rabbit pAb is validated for use in WB, ICC for the detection of c-Raf (pTyr³⁴⁰, Tyr³⁴¹).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
553009
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RRbPolyclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
553009-10T Plastic ampoule 10 t
Description
OverviewRecognizes c-Raf phosphorylated at Tyr340 and Tyr341 in HEK293 cells overexpressing c-Raf.
Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
Catalogue Number553009
Brand Family Calbiochem®
Application Data
Detection of human c-Raf, phosphorylated on Tyr340, by immunoblotting. Samples: Whole cell lysate from HEK293 cells overexpressing c-Raf left unstimulated (lane 1) or stimulated with active Src (lane 2-5). Primary antibody: PhosphoDetect™ Anti-c-Raf (pTyr340, Tyr341) Rabbit pAb (Cat. No. 553009) (1:000) pre-incubated without peptide (lanes 1, 2), with non-phosphopeptide corresponding to the immunogen (lane 3), a generic phosphoserine containing peptide (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence.
References
ReferencesYan, F., and Polk, D.B. 2001. Cancer Res. 61, 963.
Yip-Schneider, M.T., et al. 2000. Biochem. J. 351, 151.
Mason, C.S. et al. 1999. EMBO J. 18, 2137.
Bonner, T.I. et al. 1985. Mol. Cell. Biol. 5, 1400.
Moelling, K. et al. 1984. Nature 312, 558.
Rapp, U.R. et al. 1983. Proc. Natl. Acad. Sci. USA 80, 4218.
Product Information
FormLiquid
FormulationIn phosphate buffer (without Mg2+ and Ca2+), 1 mg/ml BSA, 50% glycerol, pH 7.3 (+/- 0.1).
Positive controlHEK293 cells over expressing c-Raf, phosphorylated in vitro by Src.
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunocytochemistry
Application NotesImmunoblotting (1:1000)
Immunocytochemistry (see comments)
Application CommentsThe immunogen sequence is 100% conserved in mouse and rat, but cross-reactivity has not been tested. This antibody has also been reported to work for immunocytochemistry. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr³⁴⁰ and Tyr³⁴¹, phosphorylation sites of human c-Raf (Raf-1)
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
553009-10T 04055977193411

Documentation

PhosphoDetect™ Anti-c-Raf (pTyr³⁴⁰, Tyr³⁴¹) Rabbit pAb SDS

Title

Safety Data Sheet (SDS) 

PhosphoDetect™ Anti-c-Raf (pTyr³⁴⁰, Tyr³⁴¹) Rabbit pAb Certificates of Analysis

TitleLot Number
553009

References

Reference overview
Yan, F., and Polk, D.B. 2001. Cancer Res. 61, 963.
Yip-Schneider, M.T., et al. 2000. Biochem. J. 351, 151.
Mason, C.S. et al. 1999. EMBO J. 18, 2137.
Bonner, T.I. et al. 1985. Mol. Cell. Biol. 5, 1400.
Moelling, K. et al. 1984. Nature 312, 558.
Rapp, U.R. et al. 1983. Proc. Natl. Acad. Sci. USA 80, 4218.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision15-August-2007 RFH
ApplicationImmunoblotting (1:1000)
Immunocytochemistry (see comments)
Application Data
Detection of human c-Raf, phosphorylated on Tyr340, by immunoblotting. Samples: Whole cell lysate from HEK293 cells overexpressing c-Raf left unstimulated (lane 1) or stimulated with active Src (lane 2-5). Primary antibody: PhosphoDetect™ Anti-c-Raf (pTyr340, Tyr341) Rabbit pAb (Cat. No. 553009) (1:000) pre-incubated without peptide (lanes 1, 2), with non-phosphopeptide corresponding to the immunogen (lane 3), a generic phosphoserine containing peptide (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence.
DescriptionRabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~74 KDa c-Raf protein phosphorylated on Tyr340/341.
BackgroundThe raf family of proto-oncogenes consists of three members: A-raf-1, B-raf, and c-raf-1 which show the greatest degree of homology in the carboxy-terminal half of the molecule. In the case of c-raf-1 this region has been shown to have protein (serine/threonine) kinase activity. C-raf was first identified in an oncogeneic form, v-raf, which is the oncogene of the transforming virus MSV. The raf-1 gene is the most studied of the three and encodes a protein with an apparent molecular mass of ~74 kDa. Raf is widely expressed and plays an important role in cell proliferation and differentiation. Raf relays signals related to the activation of growth factor receptor to the activation of MAPK and utlimately to the nucleus. Activation of raf itself is dependent on tyrosine (341) phosphorylation. Tyrosine phosphorylation is accomplished by Src tyrosine kinase.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr³⁴⁰ and Tyr³⁴¹, phosphorylation sites of human c-Raf (Raf-1)
IsotypeIgG
Specieshuman, mouse, rat
Positive controlHEK293 cells over expressing c-Raf, phosphorylated in vitro by Src.
FormLiquid
FormulationIn phosphate buffer (without Mg2+ and Ca2+), 1 mg/ml BSA, 50% glycerol, pH 7.3 (+/- 0.1).
Preservative≤0.1% sodium azide
CommentsThe immunogen sequence is 100% conserved in mouse and rat, but cross-reactivity has not been tested. This antibody has also been reported to work for immunocytochemistry. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesYan, F., and Polk, D.B. 2001. Cancer Res. 61, 963.
Yip-Schneider, M.T., et al. 2000. Biochem. J. 351, 151.
Mason, C.S. et al. 1999. EMBO J. 18, 2137.
Bonner, T.I. et al. 1985. Mol. Cell. Biol. 5, 1400.
Moelling, K. et al. 1984. Nature 312, 558.
Rapp, U.R. et al. 1983. Proc. Natl. Acad. Sci. USA 80, 4218.