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AP1062 PhosphoDetect™ Anti-PDH-E1α (pSer²⁹³) Rabbit pAb

This PhosphoDetect™ Anti-PDH-E1α (pSer²⁹³) Rabbit pAb is validated for use in Immunoblotting, IP, ICC for the detection of PDH-E1α (pSer²⁹³).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Pyruvate Dehydogenase pSer²⁹³ Rabbit pAb

AP1062
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Overview

Key Spec Table

Species ReactivityHostAntibody Type
H, M, Pm, RRbPolyclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
AP1062-50UL Plastic ampoule 50 ul
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AP1062-5MG Plastic ampoule 5 mg
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Description
OverviewRecognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser293 in HEK293 cells.
Catalogue NumberAP1062
Brand Family Calbiochem®
SynonymsAnti-Pyruvate Dehydogenase pSer²⁹³ Rabbit pAb
Application Data
Detection of human PDH-E3α, phosphorylated at Ser293, by immunocytochemistry. Samples: HEK293 cells treated with DCA (bottom panels) or left untreated (top panels) and subsequently fixed with 4% paraformaldehyde. Primary antibody: PhosphoDetect™ Anti-PDH-E1α (pSer293) Rabbit pAb (Cat. No. AP1062) (1:500). Detection: fluorescence (red) using DAPI to counterstain the nuclei (blue) and AlexaFluor®-Phalloidin to counterstain actin filaments (green).

Detection of human PDH-E1α, phosphorylated at Ser293, by immunoprecipitation, followed by immunoblotting. Samples: Whole cell lysate (375 µg) from HEK293 cells. Antibody for IP: PhosphoDetect™ Anti PDH-E1α (pSer293) Rabbit pAb (Cat. No. AP1062) (1:50). . Antibodyfor IB: Anti-PDH-E1α (pSer293 Rabbit pAb (Cat. No. AP1062) (1:1000) Detection: chemiluminescence.

Detection of human PDH-E1α, phosphorylated at Ser293, by immunoblotting. Samples: Whole cell lysate from HEK293 cells left untreated (lane 1) and HEK293 cells treated with dichloroacetate. Primary antibody: PhosphoDetect™ Anti PDH-E1α (pSer293) Rabbit pAb (Cat. No. AP1062) (1:10,000). Detection: chemiluminescence.
References
ReferencesSeifert, F., et al. 2007. Biochemistry 21, 6277.
Lee, J., et al. 2007. Mol. Cell Prot. 4, 669.
Korotchkina, L.G., et al. 2001. J. Biol. Chem. 40, 37223.
Product Information
FormLiquid
FormulationIn 150 mM NaCl, 100 mM Tris-Glycine, pH 7.4.
Positive controlHEK293 cells
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunocytochemistry
Immunoprecipitation
Application NotesImmunoblotting (1:10,000)
Immunoprecipitation (1:50)
Immunocytochemistry (1:500)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser 293 phosphorylation site of human PDH-E1α
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
  • Primate
  • Rat
Antibody TypePolyclonal Antibody
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Global Trade Item Number
Catalogue Number GTIN
AP1062-50UL 04055977227765
AP1062-5MG 04053252046513