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NB13 PhosphoDetect™ Anti-Ca2+/Calmodulin Kinase II α-Subunit (pThr²⁸⁶) (Ab-2) Mouse mAb (22B1)

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NB13
  
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Overview

Replacement Information

Key Spec Table

Host
M
Description
Overview

This product has been discontinued.





Recognizes the ~50 kDa α subunit of CaM kinase II phosphorylated at Thr286. Does not cross-react with unphosphorylated α subunit of CaM kinase II or with the β subunit in either phosphorylation state.
Catalogue NumberNB13
Brand Family Calbiochem®
Application Data
Detection of rat Ca2+/calmodulin kinase II α-subunit, phosphorylated on Thr286, by immunoblotting. Samples: Whole tissue extract from rat brain, unphosphorylated (lane 1) and phosphorylated (lane 2). Primary antibody: PhosphoDetect™ Anti-Ca2+/Calmodulin Kinase II α-Subunit (pThr286) (Ab-2) Mouse mAb (22B1) (Cat. No. NB13) (1:2000). Detection: chemiluminescence.
References
ReferencesMayford, M., et al. 1996. Science 274, 1678.
Lisman, J. 1994. Trends Neurosci. 17, 406.
Ochiishi, T., et al. 1994. Brain Res. 659, 179.
Hanson, P.I. and Schulman, H. 1992. Annu. Rev. Biochem. 61, 559.
Silva, A.J., et al. 1992. Science 257, 201.
Erondu, N.E. and Kennedy, M.B. 1985. J. Neurosci. 5, 3270.
Product Information
FormLiquid
FormulationIn PBS.
Preservative≤0.1% sodium azide
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunofluorescence
Application NotesELISA (see comments)
Immunoblotting (1:2000)
Immunofluorescence (1:100)
Application CommentsDoes not cross-react with the non-phosphorylated α subunit of CaM kinase II or the 60 kDa β subunit in either phosphorylation state. In rat hippocampal cells, this antibody labels cell somas and neuropil, while nuclei are only lightly stained by immunofluorescence. This antibody has been reported to work for ELISA. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic phosphopeptide (MHRQEpTVDCLKKFN) corresponding to amino acids 281-294 surrounding the Thr²⁸⁶ phosphorylation site of mouse and rat CaM kinase II α subunit
ImmunogenMouse and Rat
Clone22B1
HostMouse
IsotypeIgG₁
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
NB13 0

Documentation

PhosphoDetect™ Anti-Ca2+/Calmodulin Kinase II α-Subunit (pThr²⁸⁶) (Ab-2) Mouse mAb (22B1) Certificates of Analysis

TitleLot Number
NB13

References

Reference overview
Mayford, M., et al. 1996. Science 274, 1678.
Lisman, J. 1994. Trends Neurosci. 17, 406.
Ochiishi, T., et al. 1994. Brain Res. 659, 179.
Hanson, P.I. and Schulman, H. 1992. Annu. Rev. Biochem. 61, 559.
Silva, A.J., et al. 1992. Science 257, 201.
Erondu, N.E. and Kennedy, M.B. 1985. J. Neurosci. 5, 3270.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision05-September-2007 RFH
ApplicationELISA (see comments)
Immunoblotting (1:2000)
Immunofluorescence (1:100)
Application Data
Detection of rat Ca2+/calmodulin kinase II α-subunit, phosphorylated on Thr286, by immunoblotting. Samples: Whole tissue extract from rat brain, unphosphorylated (lane 1) and phosphorylated (lane 2). Primary antibody: PhosphoDetect™ Anti-Ca2+/Calmodulin Kinase II α-Subunit (pThr286) (Ab-2) Mouse mAb (22B1) (Cat. No. NB13) (1:2000). Detection: chemiluminescence.
DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with HL-1 mouse myeloma cells. Recognizes the ~50 kDa α subunit of CaM kinase II phosphorylated on Thr286.
BackgroundThe Ca2+/calmodulin-dependent kinase II (CaMKII) is a serine-threonine protein kinase which has been shown to phosphorylate a number of neural substrates including synapsin I, nitric oxide synthase, tryptophan and tyrosine hydroxylase, and the inositol 1,4,5-triphosphate receptors (IP3Rs). CaMKII therefore regulates a broad array of functions including the metabolism of carbohydrates, lipids, and amino acids, neurotransmitter synthesis and release, ion channel and cytoskeletal function, and gene expression. CaMKII is a heterooligomer consisting of two distinct but related isoforms of α and β which are abundantly expressed in neurons of the neocortex, hippocampus, amygdala, and basal ganglia. Following a brief exposure to Ca2+, CaMKII can be converted to a Ca2+-independent form via autophosphorylation of threonine-286, suggesting that CaMKII may play an important physiological role in memory formation. Indeed, both mutant mice deficient in CaMKII, and animals expressing an activated calcium-independent form of CaMKII shows deficiencies in hippocampal long-term potentiation (LTP).
HostMouse
Immunogen speciesMouse and Rat
Immunogena synthetic phosphopeptide (MHRQEpTVDCLKKFN) corresponding to amino acids 281-294 surrounding the Thr²⁸⁶ phosphorylation site of mouse and rat CaM kinase II α subunit
Clone22B1
IsotypeIgG₁
Speciesrat
FormLiquid
FormulationIn PBS.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsDoes not cross-react with the non-phosphorylated α subunit of CaM kinase II or the 60 kDa β subunit in either phosphorylation state. In rat hippocampal cells, this antibody labels cell somas and neuropil, while nuclei are only lightly stained by immunofluorescence. This antibody has been reported to work for ELISA. Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesMayford, M., et al. 1996. Science 274, 1678.
Lisman, J. 1994. Trends Neurosci. 17, 406.
Ochiishi, T., et al. 1994. Brain Res. 659, 179.
Hanson, P.I. and Schulman, H. 1992. Annu. Rev. Biochem. 61, 559.
Silva, A.J., et al. 1992. Science 257, 201.
Erondu, N.E. and Kennedy, M.B. 1985. J. Neurosci. 5, 3270.