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537088 PRONASE® Protease, Streptomyces griseus, Nuclease-Free

537088
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
537088-100KU Plastic ampoule 100 ku
537088-10KU Plastic ampoule 10 ku
537088-25KU Glass bottle 25 ku
537088-50KU Plastic ampoule 50 ku
537088-5KU Plastic ampoule 5 ku
537088-KU Glass bottle
Description
OverviewA non-specific protease liquefies mucins and digests proteins to free amino acids. Can be used to isolate living chondrocytes. Most active at neutral pH, but stable over wide range of pH and temperature. Note: 1KU = 1000 units.
Note: 1 KU = 1000 units.
Catalogue Number537088
Brand Family Calbiochem®
References
Product Information
CAS number9036-06-0
Activity≥70,000 PUK (proteolytic units)/g dry weight
Unit of DefinitionOne unit is defined as the amount of enzyme that will liberate a digestion product equivalent to 25 µg of tyrosine per min at 40°C, pH 7.5.
FormLyophilized
Applications
Biological Information
Physicochemical Information
ContaminantsNuclease: not detectable
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSUK9595000
Safety Information
R PhraseR: 20-36/37/38-42/43

Harmful by inhalation.
Irritating to eyes, respiratory system and skin.
May cause sensitization by inhalation and skin contact.
S PhraseS: 22-26-36

Do not breathe dust.
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Harmful
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsUnstable in solution; reconstitute just prior to use.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
537088-100KU 04055977195965
537088-10KU 04055977195958
537088-25KU 04055977195972
537088-50KU 04055977195996
537088-5KU 04055977195989
537088-KU 04053252042737

Documentation

PRONASE® Protease, Streptomyces griseus, Nuclease-Free SDS

Title

Safety Data Sheet (SDS) 

PRONASE® Protease, Streptomyces griseus, Nuclease-Free Certificates of Analysis

TitleLot Number
537088
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision17-September-2007 RFH
DescriptionA non-specific protease liquefies mucins and digests proteins to free amino acids. Can be used to isolate living chondrocytes. Most active at pH 7.0 but stable over wide ranges of pH and temperature. Has an optimal pH of 7.5.
FormLyophilized
CAS number9036-06-0
RTECSUK9595000
ContaminantsNuclease: not detectable
Activity≥70,000 PUK (proteolytic units)/g dry weight
Unit definitionOne unit is defined as the amount of enzyme that will liberate a digestion product equivalent to 25 µg of tyrosine per min at 40°C, pH 7.5.
SolubilityH₂O (10 mg/ml)
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsUnstable in solution; reconstitute just prior to use.
Toxicity Harmful