Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

539620 PP2A Inhibitor II, GST-Fusion, Human, Recombinant, E. coli - Calbiochem

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Protein Phosphatase 2A Inhibitor II, I₂PP2A

Primary Target: PP2A
539620
  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Overview

This product has been discontinued.



Recombinant, human PP2AI I2 fused to GST and expressed in E. coli. Inhibits protein phosphatase 2A (PP2A) potently with Ki ~ 25-30 nM with myelin basic protein, histone H1, and other substrates of PP2A but not with casein as a substrate. Stimulates PP1. Prevents inhibition of E-Cdk-2 by p21Cip1. Associates with B cyclin. Regulates histone binding to DNA, transcriptional activity and chromatin condensation. Undergoes phosphorylation in cells. Highly expressed in some cancers. Associates with HRX leukemia-associated fusion proteins. Occurs as fusion protein in acute undifferentiated leukemia. Does not inhibit PP1, PP2B, PP2C, or pyruvate dehydrogenase.
Catalogue Number539620
Brand Family Calbiochem®
SynonymsProtein Phosphatase 2A Inhibitor II, I₂PP2A
References
ReferencesLi, M., et al. 1996. Biochemistry 35, 6998.
Li, M., et al. 1995. Biochemistry 34, 1988.
Product Information
ATP CompetitiveN
FormLiquid
FormulationIn 50 mM Tris-HCl, 14 mM βME, 1 mM benzamidine, 1 mM EDTA, 0.1 mM PMSF, 10% glycerol, pH 7.4.
ReversibleN
Quality LevelMQ100
Applications
Biological Information
Primary TargetPP2A
Primary Target K<sub>i</sub>~ 25-30 nM
Purity≥90% by SDS-PAGE
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Cell permeableN
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
539620 0

Documentation

PP2A Inhibitor II, GST-Fusion, Human, Recombinant, E. coli - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

PP2A Inhibitor II, GST-Fusion, Human, Recombinant, E. coli - Calbiochem Certificates of Analysis

TitleLot Number
539620

References

Reference overview
Li, M., et al. 1996. Biochemistry 35, 6998.
Li, M., et al. 1995. Biochemistry 34, 1988.

Brochure

Title
Protein Phosphatases Technical Bulletin
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision30-June-2011 JSW
SynonymsProtein Phosphatase 2A Inhibitor II, I₂PP2A
DescriptionRecombinant, human PP2AI I2 fused to GST and expressed in E. coli. Inhibits PP2A with myelin basic protein, histone H1 or phosphorylase as substrates, but not with casein as the substrate. Inhibits PP2A in a noncompetitive manner (Ki = ~25-30 nM with myelin basic protein as the substrate). Does not inhibit protein phosphatase 1, protein phosphatase 2B, protein phosphatase 2C, or pyruvate dehydrogenase phosphatase.
FormLiquid
FormulationIn 50 mM Tris-HCl, 14 mM βME, 1 mM benzamidine, 1 mM EDTA, 0.1 mM PMSF, 10% glycerol, pH 7.4.
Concentration Label Please refer to vial label for lot-specific concentration
Purity≥90% by SDS-PAGE
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesLi, M., et al. 1996. Biochemistry 35, 6998.
Li, M., et al. 1995. Biochemistry 34, 1988.