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14-916M PEK, active

PEK, active MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-916M
250 µg  Also available in 10μg size as catalog # 14-916 and in 250 μg as catalog # 14-916-K.
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number14-916M
Trade Name
  • Upstate
DescriptionPEK, active
OverviewPhosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1)
References
Product Information
PresentationProtein in of 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution
Quality LevelMQ100
Applications
Key Applications
  • Kinase Assay
Biological Information
SourceN-terminal 6His-tagged, recombinant, human PEK amino acids 536 – end, expressed by baculovirus in Sf21 insect cells.
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Gene Symbol
  • PEK
  • PERK
  • EIF2AK3
Purification MethodPurified using immobilized metal affinity chromatography.
UniProt Number
Molecular Weight71 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by phosphorylation of the peptide RSRSRSRSRSRSRSR
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C
Packaging Information
Material Size250 µg
Material PackageAlso available in 10μg size as catalog # 14-916 and in 250 μg as catalog # 14-916-K.
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
14-916M 04053252556319

Related Products & Applications

Alternative Packsize

Catalogue Number Description
14-916 PEK Protein, active, 10 µg

Product Families

Kinase & Phosphatase Products

Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
Learn More >>

Categories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases