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521215 PDGF, AA Homodimer, Human, Recombinant, E. coli

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Pre-B Cell Stimulation Factor/Stromal Cell-Derived Factor-βrhPDGF-AA

521215
  
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Overview

Replacement Information

Key Spec Table

Purity
≥97% by SDS-PAGE
Description
OverviewRecombinant, human platelet derived growth factor AA (disulfide-linked dimer of two A-chain monomers) expressed in E. coli. Amino acid sequence is the long form of mature human PDGF-A chain and is identical to that of the native human nucleotide sequence except for the addition of an N-terminal methionine group. Implicated in the differentiation of cells of the oligodendrocyte lineage.
Catalogue Number521215
Brand Family Calbiochem®
SynonymsPre-B Cell Stimulation Factor/Stromal Cell-Derived Factor-βrhPDGF-AA
References
ReferencesButt, A.M., et al. 1997. J. Neurosci. Res. 48, 588.
Westermark, B., and Heldin, C.-H. 1991. Cancer Res. 51, 5087.
Betsholtz, C., et al. 1986. Nature 320, 695.
Raines, E.W., et al. 1985. Methods Enzymol. 109, 749.
Product Information
FormLyophilized
FormulationLyophilized from a sterile-filtered solution in 30% acetonitrile, 0.1% TFA.
Quality LevelMQ100
Applications
Biological Information
Biological activity1-3 ng/ml
Purity≥97% by SDS-PAGE
Physicochemical Information
ContaminantsEndotoxin: ≤1 EU/µg PDGF-AA
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage ≤ -70°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long-term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at 4°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
521215 0

Documentation

PDGF, AA Homodimer, Human, Recombinant, E. coli SDS

Title

Safety Data Sheet (SDS) 

PDGF, AA Homodimer, Human, Recombinant, E. coli Certificates of Analysis

TitleLot Number
521215

References

Reference overview
Butt, A.M., et al. 1997. J. Neurosci. Res. 48, 588.
Westermark, B., and Heldin, C.-H. 1991. Cancer Res. 51, 5087.
Betsholtz, C., et al. 1986. Nature 320, 695.
Raines, E.W., et al. 1985. Methods Enzymol. 109, 749.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision16-May-2008 RFH
SynonymsPre-B Cell Stimulation Factor/Stromal Cell-Derived Factor-βrhPDGF-AA
DescriptionRecombinant, human platelet derived growth factor AA (disulfide-linked dimer of two A-chain monomers) expressed in E. coli. Amino acid sequence is the long form of mature human PDGF-A chain and is identical to that deduced from the native human nucleotide sequence except for the addition of an N-terminal methionine group.
FormLyophilized
FormulationLyophilized from a sterile-filtered solution in 30% acetonitrile, 0.1% TFA.
Purity≥97% by SDS-PAGE
ContaminantsEndotoxin: ≤1 EU/µg PDGF-AA
Biological activity1-3 ng/ml
SolubilityReconstitute to a concentration ≥10 µg/ml with sterile 4 mM HCl containing at least 0.1% HSA or BSA.
Storage ≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long-term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at 4°C.
Toxicity Standard Handling
Merck USA index14, 7525
ReferencesButt, A.M., et al. 1997. J. Neurosci. Res. 48, 588.
Westermark, B., and Heldin, C.-H. 1991. Cancer Res. 51, 5087.
Betsholtz, C., et al. 1986. Nature 320, 695.
Raines, E.W., et al. 1985. Methods Enzymol. 109, 749.