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538384 NCK/PAK Blocking Peptide

NCK/PAK Blocking Peptide MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: TAT-PAK peptide, NCK blocking peptide

Primary Target: NCK
538384
  
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Overview

Replacement Information

Key Spec Table

Empirical Formula
C₁₂₆H₂₂₁N₅₁O₃₂S₂·11TFA·7H2O
Description
OverviewA cell-permeable synthetic peptide derived from the proline-rich region near the N-terminal of p21 activated kinase1 (PAK1) and fused with HIV TAT. Shown to selectively binds to the non-catalytic region of tyrosine kinase adaptor protein (NCK) and disrupts the cellular transport and localization of PAK. Shown to specifically inhibit endothelial cell migration and contractility (IC50 = 2 µM) and block bFGF-induced angiogenesis in a chick chorioallantoic membrane (CAM) assay. Does not exhibit any deleterious effects on endothelial cell survival and growth.

Please note that the molecular weight for this compound is batch-specific due to variable water content. Please refer to the vial label or the certificate of analysis for the batch-specific molecular weight. The molecular weight provided represents the baseline molecular weight without water.
Catalogue Number538384
Brand Family Calbiochem®
SynonymsTAT-PAK peptide, NCK blocking peptide
References
ReferencesChen, J., et al. 2015, Sci. Signal. 8, 365.
Kiosses, W., et al. 2002, Circ. Res. 90, 697.
Product Information
FormOff-white solid
FormulationSupplied as TFA salt.
Hill FormulaC₁₂₆H₂₂₁N₅₁O₃₂S₂·11TFA·7H2O
Chemical formulaC₁₂₆H₂₂₁N₅₁O₃₂S₂·11TFA·7H2O
Hygroscopic Hygroscopic
ReversibleY
Quality LevelMQ100
Applications
Biological Information
Primary TargetNCK
Primary Target IC<sub>50</sub>2 µ
Purity≥98% by HPLC
Physicochemical Information
Cell permeableY
Peptide SequenceH2N-YGRKKRRQRRRGKPPAPPMRNTSTM-C02H
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
538384 0

Documentation

NCK/PAK Blocking Peptide SDS

Title

Safety Data Sheet (SDS) 

References

Reference overview
Chen, J., et al. 2015, Sci. Signal. 8, 365.
Kiosses, W., et al. 2002, Circ. Res. 90, 697.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision24-October-2017
SynonymsTAT-PAK peptide, NCK blocking peptide
DescriptionA cell-permeable synthetic peptide derived from the proline-rich region near the N-terminal of p21 activated kinase1 (PAK1) and fused with HIV TAT. Shown to selectively binds to the non-catalytic region of tyrosine kinase adaptor protein (NCK) and disrupts the cellular transport and localization of PAK. Shown to specifically inhibit endothelial cell migration and contractility (IC50 = 2 µM) and block bFGF-induced angiogenesis in a chick chorioallantoic membrane (CAM) assay. Does not exhibit any deleterious effects on endothelial cell survival and growth.
FormOff-white solid
FormulationSupplied as TFA salt.
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₁₂₆H₂₂₁N₅₁O₃₂S₂·11TFA·7H2O
Peptide SequenceH2N-YGRKKRRQRRRGKPPAPPMRNTSTM-C02H
Purity≥98% by HPLC
SolubilityH₂O (100 mg/ml)
Storage Protect from light
-20°C
Hygroscopic
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
Toxicity Standard Handling
ReferencesChen, J., et al. 2015, Sci. Signal. 8, 365.
Kiosses, W., et al. 2002, Circ. Res. 90, 697.