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481974 NADPH-P450 Oxidoreductase, Human, Recombinant, S. frugiperda

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
481974
  
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Overview

Replacement Information
Description
Overview

This product has been discontinued.



Recombinant, human NADPH-P450 oxidoreductase expressed in S. frugiperda insect cells. A membrane bound flavoprotein containing one mole each of FAD and FMN. Transfers electrons from NADPH to the various isoforms of cytochrome P450. Typically used at ratio 1:10 to 1:2 (reductase:P450) for in vitro reconstitution assays. Suitable for in vitro drug metabolism and toxicology research including reconstituted P450-dependent biotransformation reactions.
Catalogue Number481974
Brand Family Calbiochem®
References
ReferencesShen, A.L., et al. 1989. J. Biol. Chem. 264, 7584.
Yamano, S., et al. 1989. Mol. Pharmacol. 36, 83.
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme that will oxidize 1 µmol of cytochrome <i>c</i> per min at 20°C, pH 7.7.
EC number1.6.2.4
FormLiquid
FormulationIn 10 mM potassium phosphate buffer, 1 mM DTT, 100 µM EDTA, 20% glycerol, pH 7.7.
Quality LevelMQ100
Applications
Biological Information
Purity≥90% by SDS-PAGE
Specific Activity≥20 units/mg protein
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
481974 0

Documentation

NADPH-P450 Oxidoreductase, Human, Recombinant, S. frugiperda SDS

Title

Safety Data Sheet (SDS) 

NADPH-P450 Oxidoreductase, Human, Recombinant, S. frugiperda Certificates of Analysis

TitleLot Number
481974

References

Reference overview
Shen, A.L., et al. 1989. J. Biol. Chem. 264, 7584.
Yamano, S., et al. 1989. Mol. Pharmacol. 36, 83.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision15-May-2008 RFH
DescriptionRecombinant, human NADPH-P450 oxidoreductase expressed in S. frugiperda insect cells. A membrane-bound flavoprotein containing one mole each of FAD and FMN. Transfers electrons from NADPH to the various isoforms of cytochrome P450. Typically used at ratios ranging from 1:10 to 1:2 (reductase:P450) for in vitro reconstitution assays. Suitable for in vitro drug metabolism and toxicology research including reconstituted P450-dependent biotransformation reactions.
FormLiquid
FormulationIn 10 mM potassium phosphate buffer, 1 mM DTT, 100 µM EDTA, 20% glycerol, pH 7.7.
Concentration Label Please refer to vial label for lot-specific concentration
EC number1.6.2.4
Purity≥90% by SDS-PAGE
Specific activity≥20 units/mg protein
Unit definitionOne unit is defined as the amount of enzyme that will oxidize 1 µmol of cytochrome c per min at 20°C, pH 7.7.
SolubilityAqueous buffers
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesShen, A.L., et al. 1989. J. Biol. Chem. 264, 7584.
Yamano, S., et al. 1989. Mol. Pharmacol. 36, 83.