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411492 Sigma-AldrichMouse Anti-Human IgG₄, Fc Fragment Specific (HP6025)

This Mouse Anti-Human IgG₄, Fc Fragment Specific (HP6025) is validated for use in Enzyme Immunoassay for the detection of Human IgG₄, Fc Fragment Specific.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
References
Data Sheet

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Overview

Replacement Information

Products

Catalogue Number	Packaging	Qty/Pack
411492-400UG	Plastic ampoule	400 μg	Add To Favorites Add To Favorites Added To My Favorites

Description
Catalogue Number	411492
Brand Family	Calbiochem®

References
References	Hamilton, R.G. 1990. Ann. Biol. Clin. 48, 473. Fasullo, F.J., et al. 1989. Clin. Chem. 35, 364. Jefferis, R., et al. 1986. Immunol. Letters 10, 223.

Product Information
Form	Liquid
Formulation	In PBS, pH 7.4.
Preservative	None
Quality Level	MQ100

Applications
Application References	Enzyme Immunoassay Hamilton, R.G. 1987. Clin. Chem. 33, 1707.
Key Applications	Enzyme Immunoassay
Application Notes	Enzyme Immunoassay (10 µg/ml, see application references)
Application Comments	Antibody should be titrated for optimal results in individual systems. Cross-reactivity by ELISA against human myeloma proteins: Human IgG₁: ≤0.1% Human IgG₂: ≤0.04% Human IgG₃: ≤0.02% Human IgG₄: 100% Human IgA: <0.1% Human IgE: <0.1% Human IgM: <0.1% Cross-reactivity by ELISA against chimeric antibodies: Human IgG₁: <0.01% Human IgG₂: <0.01% Human IgG₃: <0.01% Human IgG₄: 100% Human IgA: <0.01% Human IgE: <0.01% Human IgM: <0.01%

Biological Information
Clone	HP6025
Host	Mouse
Isotype	IgG
Antibody Type	Monoclonal Antibody
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Dry Ice Only
Toxicity	Standard Handling
Storage	-20°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C). Avoid more than 5 freeze/thaw cycles.

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
411492-400UG	04055977211252

Documentation

Mouse Anti-Human IgG₄, Fc Fragment Specific (HP6025) SDS

Title
Safety Data Sheet (SDS)

Mouse Anti-Human IgG₄, Fc Fragment Specific (HP6025) Certificates of Analysis

Title	Lot Number
411492	Enter Lot Number

References

Reference overview
Hamilton, R.G. 1990. Ann. Biol. Clin. 48, 473. Fasullo, F.J., et al. 1989. Clin. Chem. 35, 364. Jefferis, R., et al. 1986. Immunol. Letters 10, 223.

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	06-August-2007 RFH
Application	Enzyme Immunoassay (10 µg/ml, see application references)
Description	Purified mouse monoclonal antibody. Recognizes human IgG₄ Fc fragment.
Background	The HP series of murine human Ig-specific monoclonal antibodies was produced from documented hybridoma cell lines that were developed at the Centers for Disease Control in Atlanta, Georgia. Laboratory analysis of each monoclonal antibody lot is performed in two stages during the production of ascites. First, the cell culture medium is analyzed for the presence and relative amount of human Ig-specific antibody by ELISA before injection of cells into mice. Second, collected ascites are analyzed for potency and antibody specificity using ELISA and isoelectric focusing (IEF), often in combination with an affinity immunoblot. For more information, the Calbiochem^® technical monograph, The Human IgG Subclasses, is available free of charge.
Host	Mouse
Clone	HP6025
Isotype	IgG
Form	Liquid
Formulation	In PBS, pH 7.4.
Concentration Label	Please refer to vial label for lot-specific concentration
Preservative	None
Comments	Antibody should be titrated for optimal results in individual systems. Cross-reactivity by ELISA against human myeloma proteins: Human IgG₁: ≤0.1% Human IgG₂: ≤0.04% Human IgG₃: ≤0.02% Human IgG₄: 100% Human IgA: <0.1% Human IgE: <0.1% Human IgM: <0.1% Cross-reactivity by ELISA against chimeric antibodies: Human IgG₁: <0.01% Human IgG₂: <0.01% Human IgG₃: <0.01% Human IgG₄: 100% Human IgA: <0.01% Human IgE: <0.01% Human IgM: <0.01%
Storage	Avoid freeze/thaw -20°C
Do Not Freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C). Avoid more than 5 freeze/thaw cycles.
Toxicity	Standard Handling
References	Hamilton, R.G. 1990. Ann. Biol. Clin. 48, 473. Fasullo, F.J., et al. 1989. Clin. Chem. 35, 364. Jefferis, R., et al. 1986. Immunol. Letters 10, 223.
Application references	Enzyme Immunoassay Hamilton, R.G. 1987. Clin. Chem. 33, 1707.

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