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FCMAB313PE Milli-Mark® Anti-Nestin-PE Antibody, clone 10C2

Detect Nestin using this Milli-Mark Anti-Nestin-PE Antibody, clone 10C2 validated for use in FC.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Stem Cell Research Research Sub-Category: Neural Stem Cells Gene Symbol: NES, Nbla00170 UniProt Number: P48681
FCMAB313PE
100 tests  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFCMPhycoerythrinMonoclonal Antibody
Description
Catalogue NumberFCMAB313PE
Trade Name
  • Milli-Mark
DescriptionMilli-Mark® Anti-Nestin-PE Antibody, clone 10C2
Background InformationNestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells {Folia Biologica, 44(5):155, 1998}. Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinoma.
References
Product Information
FormatPhycoerythrin
Control
  • U251 cells
PresentationPurified mouse monoclonal IgG1κ conjugated to PE in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Quality LevelMQ100
Applications
ApplicationDetect Nestin using this Milli-Mark Anti-Nestin-PE Antibody, clone 10C2 validated for use in FC.
Key Applications
  • Flow Cytometry
Biological Information
ImmunogenFusion protein
EpitopeHuman
Clone10C2
HostMouse
SpecificityAntibody recognizes Human Nestin.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteTested and passed on human. No reactivity with mouse or rat Nestin.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryNestin is an intermediate filament protein that was first identified with a monoclonal antibody by Hockfield and McKay (1985) [PubMed 4078630]. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.
Gene Symbol
  • NES
  • Nbla00170
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P48681 # May play a role in the trafficking and distribution of IF proteins and potentially other cellular factors to daughter cells during progenitor cell division (By similarity).

SIZE: 1621 amino acids; 177439 Da

SUBUNIT: Interacts with FHOD3 (By similarity).

TISSUE SPECIFICITY: CNS stem cells.

DEVELOPMENTAL STAGE: Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.

SIMILARITY: SwissProt: P48681 ## Belongs to the intermediate filament family.
Molecular Weight177 kDa Calculated
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by flow cytometry using U251 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
Packaging Information
Material Size100 tests
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
FCMAB313PE 04053252294624

Documentation

Milli-Mark® Anti-Nestin-PE Antibody, clone 10C2 SDS

Title

Safety Data Sheet (SDS) 

Milli-Mark® Anti-Nestin-PE Antibody, clone 10C2 Certificates of Analysis

TitleLot Number
Milli-Mark Anti-Nestin-PE, clone 10C2 - 2118984 2118984
Milli-Mark Anti-Nestin-PE, clone 10C2 - 2199677 2199677
Milli-Mark#174; Anti-Nestin-PE, clone 10C2 - 2017695 2017695
Milli-Mark#174; Anti-Nestin-PE, clone 10C2 - 2045857 2045857
Milli-Mark#174; Anti-Nestin-PE, clone 10C2 - NRG1937005 NRG1937005
Milli-Mark™ Anti-Nestin-PE, -2514215 2514215
Milli-Mark™ Anti-Nestin-PE, -2524561 2524561
Milli-Mark™ Anti-Nestin-PE, -2629274 2629274
Milli-Mark™ Anti-Nestin-PE, clone 10C2 - 3193339 3193339
Milli-Mark™ Anti-Nestin-PE, clone 10C2 - 3233118 3233118
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