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14-616M MSK2 Protein, active, 250 µg

Active, N-terminal His6-tagged, recombinant, human MSK2, amino acids 2-end, for use in Kinase Assays.

14-616M
250 µg  Also available in 10 μg size (cat#14-616) and in bulk (cat#14-616-K).
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number14-616M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionMSK2 Protein, active, 250 µg
OverviewN-terminal His6-tagged, recombinant, human MSK2, amino acids 2-end
References
Product Information
Quality LevelMQ300
Applications
ApplicationActive, N-terminal His6-tagged, recombinant, human MSK2, amino acids 2-end, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
Sourceexpressed by baculovirus in Sf21 insect cells
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including CREB1 and c-fos. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.
Gene Symbol
  • RPS6KA4
  • MSK2
  • RSK-B
  • RSKB
Protein TargetMSK2
Purification MethodPurified using Ni2+/NTA-agarose. Activated using MAP Kinase 2/Erk2, active (Catalog # 14-173) and repurified using Ni2+/NTA-agarose
Target Sub-FamilyAGC
UniProt Number
UniProt SummaryFUNCTION: SwissProt: O75676 # Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB. Essential role in the control of RELA transcriptional activity in response to TNF. Phosphorylates 'Ser- 10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF) and result in the transcriptional activation of several immediate early genes, including proto- oncogenes FOS and JUN (By similarity). Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14).
COFACTOR: Magnesium.
SIZE: 772 amino acids; 85606 Da
SUBUNIT: Forms a complex with either ERK1 or ERK2 in quiescent cells which transiently dissociates following mitogenic stimulation. Also associates with MAPK14/p38-alpha. Activated RPS6KA4 associates with and phosphorylates the NF-kappa-B p65 subunit RELA.
SUBCELLULAR LOCATION: Nucleus.
SIMILARITY: SwissProt: O75676 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 2 protein kinase domains.
MISCELLANEOUS: Enzyme activity requires the presence of both kinase domains (By similarity).
Molecular Weight89.9kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by phosphorylation of 30mM Crosstide (Catalog # 12-331)
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-616) and in bulk (cat#14-616-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
14-616M 04053252924330

Documentation

Related Products & Applications

Alternative Packsize

Catalogue Number Description
14-616 MSK2 Protein, active, 10 µg

Product Families

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Categories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases