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HDIAB-34K-PMX5 MILLIPLEX MAP Human Diabetes Panel, Premixed 5 Plex Assay

The Human Diabetes, Premixed 5 Plex Panel, using the Luminex xMAP technology, enables the simultaneous analysis of 5 protein biomarkers in human serum, plasma and cell culture samples.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Metabolism Research Sub-Category: Obesity, Diabetes, Diabetes - Type 1, Diabetes - Type 2
HDIAB-34K-PMX5
96-well plate  
Purchase on Sigma-Aldrich

Overview

Key Spec Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
C-Peptide GLP-1 (Active) Glucagon Insulin Leptin HMplexLuminex xMAP
Description
Catalogue NumberHDIAB-34K-PMX5
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP Human Diabetes Panel, Premixed 5 Plex Assay
OverviewEMD Millipore’s MILLIPLEX® MAP Human Diabetes Premixed Magnetic Bead Panel is a 5-plex kit to be used for the simultaneous quantification of the following analytes in serum, plasma or culture supernatant samples: C-Peptide, GLP-1 (Active), Glucagon, Insulin, Leptin.

MILLIPLEX® MAP offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. In addition to the assay characteristics listed in the protocol, other performance criteria evaluated during the validation process include: cross-reactivity, dilution linearity, kit stability, and sample behavior (e.g. detectability and stability). Each panel and kit meets stringent manufacturing criteria to ensure batch-to-batch reproducibility.

Each MILLIPLEX® MAP panel and kit includes:
• Quality controls (QCs) provided to qualify assay performance

• Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency

• Optimized serum matrix to mimic native analyte environment

• Detection antibody cocktails designed to yield consistent analyte profiles within panel


MILLIPLEX® MAP offers you:
o The ability to choose any combination of analytes from our panel of seven analytes to design a custom kit that better meets your needs.
o A convenient “all-in-one” box format that gives you the assurance that you will have all the necessary reagents you need to run your assay.

EMD Millipore’s MILLIPLEX® MAP Human Diabetes Premixed Magnetic Bead Panel is part of the most versatile system available for metabolic hormone research. From our single to multiplex biomarker solutions, we partner with you to design, develop, analytically validate and build the most comprehensive library available for protein detection and quantitation.
Background InformationType I Diabetes is an autoimmune disease (or a viral attack) that destroys pancreatic β-cells leaving the subject unable to produce insulin (usually early onset). Type II Diabetes is a metabolic disorder caused by obesity and a sedentary lifestyle (usually later onset). If the body is unable to produce sufficient levels of insulin, then Glucose levels will rise and in time, can cause heart disease, stroke, kidney failure and dementia, to name a few. The five analytes included in this kit are: insulin, glucagon, leptin, glucagon-like peptide (GLP-1 active), and C-Peptide (connecting peptide).

The MILLIPLEX® MAP Human Diabetes Premixed Magnetic Bead Panel enables you to focus on the relevance and therapeutic potential of insulin, glucagon, leptin, GLP-1 (Active), and C-Peptide in your research model. Coupled with the Luminex xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of multiple analytes simultaneously, which can dramatically improve productivity.
Product Information
Detection methodLuminex xMAP
ConfigurationPremixed
Precision, %
  • C-Peptide: Intra-assay <10% CV Inter-assay <15%
  • GLP-1 (Active): Intra-assay <10% CV Inter-assay <20% CV
  • Glucagon: Intra-assay <10% CV Inter-assay <15% CV
  • Insulin: Intra-assay <10% CV Inter-assay <15% CV
  • Leptin: Intra-assay <10% CV Inter-assay <15% CV
Panel TypeMAGNETIC Metabolism
Quality LevelMQ200
Applications
ApplicationThe Human Diabetes, Premixed 5 Plex Panel, using the Luminex xMAP technology, enables the simultaneous analysis of 5 protein biomarkers in human serum, plasma and cell culture samples.
Key Applications
  • Multiplexing
Application Notes• This kit is a 5-plex, premixed bead kit.
• Two of the analytes require addition of DPPIV inhibitor (for GLP-1 active) and Aprotinin (for Glucagon) to the serum or plasma samples. Refer to the sample collection and storage section in the protocol for details.
• The protocol recommends serum or plasma samples be run neat. Tissue/cell culture samples may require a dilution with an appropriate control medium prior to assay. • For optimal sensitivity use an overnight (16 - 18 hour) incubation.
• Each well requires 25 uL of sample, standard or control.
Biological Information
SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity
  • Human
Analytes Available
  • C-Peptide
  • GLP-1 (Active)
  • Glucagon
  • Insulin
  • Leptin
Assay PanelEverything you need in a single kit.
Cross ReactivityThere was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
Accuracy
  • C-Peptide 92%
  • GLP-1 (Active) 86%
  • Glucagon 91%
  • Insulin 91%
  • Leptin 94%
Physicochemical Information
Sensitivity
  • MinDC+2SD
  • C-Peptide: 16.4 pg/mL
  • GLP-1 (Active): 2.1 pg/mL
  • Glucagon: 15.8 pg/mL
  • Insulin: 32.7 pg/mL
  • Leptin: 65.9 pg/mL
Linearity of DilutionContact technical support for this information
Standard Curve Range
  • C-Peptide: 27.4 - 20,000 pg/mL
  • GLP-1 (active): 2.7 - 2,000 pg/mL
  • Glucagon: Approximately 13.7 - 10,000 pg/mL (Refer to QC analysis sheet for exact concentration)
  • Insulin: 137 - 100,000 pg/mL
  • Leptin: 137 - 100,000 pg/mL
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Global Trade Item Number
Catalogue Number GTIN
HDIAB-34K-PMX5 04055977310542