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HC5B9-140K MILLIPLEX® Human Complement C5b-9 - Singleplex

MILLIPLEX® Human Complement C5b-9 – Singleplex Magnetic Bead Panel is a Singleplex kit to be used for the quantification of the following analyte in serum, plasma, or cell culture supernatant samples: C5b-9 (Membrane Attack Complex, Terminal Complement Complex).

HC5B9-140K
96-well plate  96 well plate
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberHC5B9-140K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX® Human Complement C5b-9 - Singleplex
OverviewMILLIPLEX® offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. In addition to the assay characteristics listed in the protocol, other performance criteria evaluated during the verification process include: cross-reactivity, dilution linearity, kit stability, and sample behavior (e.g. detectability and stability).

Each MILLIPLEX® panel and kit includes:
• Quality controls (QCs) to qualify assay performance
• Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency
• Optimized serum matrix to mimic native analyte environment in serum and plasma samples
• Detection antibody cocktails designed to yield consistent analyte profiles within panel

In addition, each panel and kit meet stringent manufacturing criteria to ensure batch to-batch reproducibility. MILLIPLEX® Human Complement C5b-9 – Singleplex Magnetic Bead Panel is part of the most versatile system available for complement factor research. From our single to multiplex biomarker solutions, we partner with you to design, develop, analytically verify, and build the most comprehensive library available for protein detection and quantitation.
Background InformationThe complement system consists of many plasma proteins that assist, or complement, the ability of phagocytic cells and antibodies to clear pathogens. Although it is part of the innate immune system, it can be recruited and activated by the adaptive immune system. The complement proteins and glycoproteins that are synthesized primarily by hepatocytes normally circulate as inactive pro-proteins. Once stimulated the result of the cascade is a massive amplification of response and activation of the membrane attack complex (C5b-9), which forms a transmembrane pore causing osmotic lysis of target cells. The complement pathway may also form soluble C5b-9, as well as intermediate isoforms, stabilized by regulatory proteins. These soluble complexes are unable to insert into the lipid membrane, and their role in complement pathways is an area of ongoing research.
References
Product Information
Detection methodLuminex xMAP
ConfigurationSingle Plex
Precision, %
  • Intra-assay: <15%
  • Inter-assay: <15%
Panel TypeMAGNETIC Cytokines/Chemokines
Quality LevelMQ200
Applications
ApplicationMILLIPLEX® Human Complement C5b-9 – Singleplex Magnetic Bead Panel is a Singleplex kit to be used for the quantification of the following analyte in serum, plasma, or cell culture supernatant samples: C5b-9 (Membrane Attack Complex, Terminal Complement Complex).
Key Applications
  • Multiplexing
Application Notes• This is an overnight or two-hour incubation assay.
• Each assay requires 25 µL of diluted plasma or serum, or 25 μL cell culture supernatant per well.
• Complement activation can occur in samples that are not handled properly. Proper sample collection, handling, and storage is necessary to achieve accurate assay results.
• Serum samples will typically produce higher complement values. EDTA Plasma is recommended to achieve the most accurate complement values.
Biological Information
Species Reactivity
  • Human
Analytes Available
  • Complement C5b-9
Cross ReactivityCross-Reactivity between C5b-9 and other complement proteins found in MILLIPLEX® Human Complement Expanded panel 1 (Cat. No. HCMPEX1-19K) and MILLIPLEX® Human Complement Panel 2 (Cat. No. HCMP2MAG-19K) was tested. There was no or negligible cross-reactivity between the antibody pair in this panel for any of the analytes tested.
Accuracy
  • 120% overnight assay, 100% same day assay
Physicochemical Information
Sensitivity
  • 0.16 ng/mL overnight assay, 0.21 ng/mL same day assay
Standard Curve Range
  • 0.6 ng/mL - 450 ng/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Material Package96 well plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
HC5B9-140K 04065270109408

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