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IPFL00010 Immobilon®-FL PVDF Membrane

1 roll, 27 cm x 3.75 m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.

Immobilon®-FL PVDF Membrane MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Western Blotting, Dot Blot
IPFL00010
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Overview

Replacement Information

Key Spec Table

Key Applications
WB, DB
Description
Catalogue NumberIPFL00010
Trade Name
  • Immobilon
DescriptionImmobilon®-FL PVDF Membrane
Background InformationOverview
- Millipore offers four different Immobilon PVDF membranes, each optimized for different protein blotting applications.
Immobilon PVDF membranes provide many advantages compared to nitrocellulose. They won’t crack or curl, and they can be cut without fracturing. They also have low background, broad solvent compatibility, and superior staining capabilities. In addition, they can be reprobed multiple times.

Immobilon-P Transfer Membrane:
- 45 µm pore size Recommended for most western blotting, especially proteins greater than 20 kDa
- Millipore's Rapid Immunodetection Protocol reduces detection times by up to 2 hours by eliminating membrane blocking and several wash steps with no loss of sensitivity or specificity

Immobilon-E Membrane:
- the only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step.
- 45 µm pore size recommended for most western blotting, especially proteins greater than 20 kDa

Blotting Sandwiches:
- When you need to process multiple blots, blotting sandwiches are a convenient, time-saving choice. Our sandwiches include sheets of Immobilon-P membrane interleaved with pre-cut sheets of chromatography-grade blotting filter paper and are compatible with any standard wet transfer device.

Blotting Filter Paper:
- Chromatography-grade blotting filter paper pre-cut to the most popular western blotting sizes.

Immobilon-PSQ Membrane:
- 0.2 µm pore size and a large internal structure
- Higher protein adsorption and sequencing yields than other membranes
- Recommended for blotting proteins less than 20 kDa
- Prevents blow-through of low molecular weight proteins

Immobilon-FL Membrane:
- The first transfer membrane optimized for fluorescence applications
- Extremely low background improves sensitivity of all fluorescence detection protocols
- Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
- Ideal for multiplexing and chemifluorescence applications

Immobilon® PVDF membranes are offered in four types, each optimized for a different protein blotting application. Convenient blotting sandwiches feature pre-cut sheets of membrane and blotting filter paper. Immobilon® PVDF membranes have high protein adsorption, so you won’t lose proteins during transfer or reprobing. The open pore structure makes it easy to access bound proteins and remove unbound probes. In addition, Immobilon® PVDF membranes optimized for fluorescent blots dramatically increase signal-to-noise ratios for high sensitivity in quantitative, multiplexing applications.

Features & Benefits:
- Won’t crack, curl or fracture when cut
- Low background
- Superior staining capabilities
- Can be reprobed multiple times

Applications:
- Western Blotting, Immunoblotting, Dot Blotting, Protein Sequencing;
- Compatible with Chemiluminescent, Fluorescent, Radioactive, Chromogenic, and Chemifluorescent Detection
- Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
References
Product Information
Detection methodFluorescent Chemifluorescence Chromogenic Chemiluminescence 125I Fluorescent
HS Code3921 19 00
Compatible Stains
  • All fluorescence dyes
  • Coomassie brilliant blue
  • Amido black
  • Ponceau-S red
  • CPTS
  • Sypro® ruby
Filter CodeIPFL
Filter ColorWhite
Quality LevelMQ400
Applications
Application1 roll, 27 cm x 3.75 m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.
Key Applications
  • Western Blotting
  • Dot Blot
Biological Information
BSA Adsorption205 µg/cm²
Goat IgG Adsorption300 µg/cm²
Insulin Adsorption155 µg/cm²
WettabilityHydrophobic
Physicochemical Information
Pore Size0.45 µm
Dimensions
Filter SurfacePlain
Filter Dimensions27 cm x 3.75 m
Materials Information
Chemistry
  • Hydrophobic Polyvinylidene Fluoride (PVDF)
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Packaging Information
Material Size1
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
IPFL00010 04053252669439

Documentation

Immobilon®-FL PVDF Membrane SDS

Title

Safety Data Sheet (SDS) 

Brochure

Title
SNAP i.d. 2.0 System Brochure
Western Blotting Tools

Technical Info

Title
Antibody recovery and reuse in the SNAP i.d.®2.0 immunodetection system
Protein Blotting Handbook: 6th Edition

Posters

Title
Low Background Membrane for Fluorescent Protein Detection in Western Blotting

User Guides

Title
Immobilon® Block Noise Cancelling Reagents
Immobilon®-FL Transfer Membrane User Guide
User Guide - Immobilon® FL Transfer Membrane