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EZHIL8-100K Human IL-8 Conferma™ ELISA

This Human Interleukin-8 (IL-8) ELISA kit is used for the quantification of Human IL-8 in serum, plasma, or culture media samples. One kit is sufficient to measure 37 unknown samples in duplicate.

Human IL-8 Conferma™ ELISA MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: ELISA Detection Methods: Colorimetric Research Category: Inflammation & Immunology, Immune Response, Metabolism Research Sub-Category: Cytokines & Cytokine Receptors, Immune Response, Inflammation, Metabolism
EZHIL8-100K
96-well strip plate  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
IL-8 HELISAColorimetric
Description
Catalogue NumberEZHIL8-100K
DescriptionHuman IL-8 Conferma™ ELISA
Background InformationIL-8 is secreted by mononuclear macrophages, neutrophils, eosinophils, T lymphocytes, epithelial cells, and fibroblasts. It functions as a chemotactic factor by guiding the neutrophils to the site of infection. IL-8 also participates with other cytokines in the proinflammatory signaling cascade. The protein is implicated in numerous pathologies including, bacterial, cancer, cardiovascular and viral diseases.
References
Product Information
Detection methodColorimetric
HS Code3002 15 90
Precision, %
  • Inter-assay: <4.7% CV
  • Intra-assay: <4.0% CV
Quality LevelMQ200
Applications
ApplicationThis Human Interleukin-8 (IL-8) ELISA kit is used for the quantification of Human IL-8 in serum, plasma, or culture media samples. One kit is sufficient to measure 37 unknown samples in duplicate.
Key Applications
  • ELISA
Application Notes3 hour 45 minute assay, room temperature, 50µl of neat serum or plasma is recommened.
Biological Information
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Analytes Available
  • IL-8
Entrez Gene Number
Entrez Gene SummaryIL-8 is secreted by mononuclear macrophages, neutrophils, eosinophils, T lymphocytes, epithelial cells, and fibroblasts. It functions as a chemotactic factor by guiding the neutrophils to the site of infection. IL-8 also participates with other cytokines in the proinflammatory signaling cascade. The protein is implicated in numerous pathologies including, bacterial, cancer, cardiovascular and viral diseases.
Gene Symbol
  • IL-8
  • NAF
  • GCP1
  • LECT
  • LUCT
  • NAP1
  • GCP-1
  • LYNAP
  • MDNCF
  • MONAP
  • NAP-1
  • SCYB8
UniProt Number
UniProt SummaryIL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
Accuracy
  • Serum 70%-105% Av 94%
  • Plasma 77%-102% Av 85%
Physicochemical Information
Linearity of DilutionSerum Linearity: 94-106% Serum Parallelism: 109% Plasma Linearity: 105%
Standard Curve Range
  • 0.82pg/ml-200pg/ml
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • For research use only. Not for use in diagnostic procedures.
Storage and Shipping Information
Packaging Information
Material Size96-well strip plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
EZHIL8-100K 04065265645669