Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

100025 Half FRASER (Demi FRASER) broth (base) with antibiotics

acc. ISO 11290 GranuCult® prime

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
ph Value: 7.0 - 7.4 (55 g/l, H₂O, 25 °C) (after autoclaving) Solubility: 55 g/l
100025
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

ph ValueSolubility
7.0 - 7.4 (55 g/l, H₂O, 25 °C) (after autoclaving)55 g/l

Products

Catalogue NumberPackaging Qty/Pack
1000250500 Plastic bottle 500 g
1000255000 Plastic drum 5 kg
1000259010 Plastic drum 10 kg
Description
Catalogue Number100025
OverviewGranuCult™ is our new brand name for our existing granulated dehydrated culture media which apply to new EN ISO 11133:2014 and various other ISO standards and FDA-BAM / USDA-FSIS methods for testing of food and water samples. All these media are currently being updated to be fully compliant to the latest version of these standards and regulations with respect to composition and quality control and will be available shortly.
References
Product Information
HS Code3821 00 00
Quality LevelMQ200
Applications
Biological Information
Physicochemical Information
pH value7.0 - 7.4 (55 g/l, H₂O, 25 °C) (after autoclaving)
Solubility55 g/l
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Storage class10 - 13 Other liquids and solids
WGKWGK 3 highly hazardous to water
Disposal3
Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.
Safety Information
Product Usage Statements
Storage and Shipping Information
StorageStore at +15°C to +25°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Appearance (clearness)clear
Appearance (color)yellowish-brown
pH-value (25 °C)7.0 - 7.4
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Mixed inoculum of Listeria, E.coli and E.faecalis
Additionaly monoculture of E.coli and E.faecalis
Afterwards confirmation on ALOA®-Medium resp. Tryptic Soy agar
Inoculum on reference medium (Listeria monocytogenes ATCC 13932 (WDCM 00021))≤ 100
Inoculum on reference medium (Listeria monocytogenes ATCC 35152 (WDCM 00109))≤ 100
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013))≥ 1000
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012))≥ 1000
Inoculum on reference medium (Enterococcus faecalis ATCC 19433 (WDCM 00009))≥ 1000
Inoculum on reference medium (Enterococcus faecalis ATCC 29212 (WDCM 00087))≥ 1000
Growth on ALOA® medium (Listeria monocytogenes ATCC 13932 (WDCM 00021))> 10 colonies
Growth on ALOA® medium(Listeria monocytogenes ATCC 35152 (WDCM 00109))> 10 colonies
Colonies (Listeria monocytogenes ATCC 13932 (WDCM 00021))blue green colonies with opaque halo
Colonies(Listeria monocytogenes ATCC 35152 (WDCM 00109))blue green colonies with opaque halo
Growth on Tryptic soy agar (Escherichia coli ATCC 25922 (WDCM 00013))total inhibition
Growth on Tryptic soy agar (Escherichia coli ATCC 8739 (WDCM 00012))total inhibition
Growth on Tryptic soy agar (Enterococcus faecalis ATCC 29212 (WDCM 00087))< 100 colonies
Growth on Tryptic soy agar (Enterococcus faecalis ATCC 19433 (WDCM 00009))< 100 colonies
Incubation:
Half Fraser Broth: 24 ± 2 hours at 30 ± 1°C
Confirmation on ALOA®-Medium: 44 ± 4 hours at 37 ± 1°C
Confirmation on Tryptic Soy Agar: 24 ± 3 hours at 37 ± 1°C
Global Trade Item Number
Catalogue Number GTIN
1000250500 04027269049157
1000255000 04027269984717
1000259010 04027269222451