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115498 HPTLC Silica gel 60 RP-18 F₂₅₄s with concentrating zone 20 x 2.5 cm

115498
Purchase on Sigma-Aldrich

Overview

Replacement Information
Replacement InformationIn North America 1.15498.0001 replaces and is identical to 15498-6

Products

Catalogue NumberPackaging Qty/Pack
1154980001 Fibre case 25 units
Description
Catalogue Number115498
Replaces15498-6; 15498
DescriptionHPTLC Silica gel 60 RP-18 F₂₅₄s with concentrating zone 20 x 2.5 cm
OverviewThese plates allow users to quickly and easily apply any kind of sample, even large volumes of diluted samples. Our concentrating zone plates are based on different adsorption properties of two adsorbents. The first is a large pore concentrating adsorbent where the samples are applied; the second is a selective layer for separation. Regardless of the spots’ shape, size, or position, the sample always concentrates as a narrow band where the two adsorbents overlap and where the separation starts. These plates allow users to quickly and easily apply any kind of sample, even large volumes of diluted samples.
Product Information
HS Code3822 19 90
Quality LevelMQ100
Applications
ApplicationGlass HPTLC Silica gel 60 RP-18 F₂₅₄s with concentrating zone 20 x 2.5 cm plates. Silica gel HPTLC plates size 20 x 10 cm, 25 sheets.
Safety Information according to GHS
Storage class10 - 13 Other liquids and solids
Storage and Shipping Information
StorageStore at +2°C to +30°C.
Specifications
Specific surface area (according to BET; 5-Pt. measurement)480 - 540 m²/g
Pore volume (N₂-isotherm)0.74 - 0.84 ml/g
d 50 (laser diffraction, size distribution)5 - 7 µm
Layer thickness150 - 200 µm
Deviation of layer thickness per plate≤ 35 µm
layer thickness concentrating zone70 - 110 µm
Fluctuation in the thickness of the concentration zone≤ 35 µm
Chromatographic testings:
A) cholesterin test
hRf-values
- cholesterin, cholesterin test

11 - 23
B) Steroide test
- methyltestosterone, steroid test20 - 40
- Reichstein S, steroid test50 - 70
- hydrocortisone, steroid test65 - 85
Typical value determined on a activated plate (120°C, 15 min)
Eluent a) acetone/ water (95/5 v/v)
Eluent b) acetonitrile/ water (70/30 v/v)
Global Trade Item Number
Catalogue Number GTIN
1154980001 04022536160672