Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

14-679M HIPK1 Protein, active, 250 µg

Active, N-terminal 6His-tagged, recombinant, human HIPK1, amino acids 158-555. For use in Kinase Assays.

14-679M
250 µg  Also available in 10 μg size (cat#14-679) and in bulk (cat#14-679-K).
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number14-679M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionHIPK1 Protein, active, 250 µg
OverviewN-terminal 6His-tagged, recombinant, human HIPK1, amino acids 158-555.
References
Product Information
Quality LevelMQ300
Applications
ApplicationActive, N-terminal 6His-tagged, recombinant, human HIPK1, amino acids 158-555. For use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceHIPK1 expressed by baculovirus in Sf21 cells.
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene belongs to the Ser/Thr family of protein kinases and HIPK subfamily. It phosphorylates homeodomain transcription factors and may also function as a co-repressor for homeodomain transcription factors. Alternative splicing results in four transcript variants encoding four distinct isoforms.
Gene Symbol
  • HIPK1
  • MGC33446
  • KIAA0630
  • Nbak2
  • MGC26642
  • MGC33548
  • Myak
Protein TargetHIPK1
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyCMGC
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q86Z02 # May play a role as a corepressor for homeodomain transcription factors. Phosphorylates DAXX in response to stress, and mediates its translocation from the nucleus to the cytoplasm. May be involved in malignant squamous cell tumor formation.
SIZE: 1210 amino acids; 130843 Da
SUBUNIT: Interacts with Nkx1-2 and Nkx2-5 (By similarity). Interacts with DAXX and TP53.
SUBCELLULAR LOCATION: Nucleus (By similarity). Cytoplasm (By similarity). Note=Predominantly nuclear.
TISSUE SPECIFICITY: Ubiquitously expressed with highest levels in skeletal muscle and heart. Overexpressed in breast cancer cell lines.
PTM: Autophosphorylated. Phosphorylated and activated by JNK1.
SIMILARITY: SwissProt: Q86Z02 ## Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. HIPK subfamily. & Contains 1 protein kinase domain.
Molecular Weight49.6kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by phosphorylation of MBP (13-104).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C from date of shipment
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-679) and in bulk (cat#14-679-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
14-679M 04053252924965

Documentation

Related Products & Applications

Alternative Packsize

Catalogue Number Description
14-679 HIPK1 Protein, active, 10 µg

Product Families

Kinase & Phosphatase Products

Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
Learn More >>

Categories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases