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368043 Granzyme B, Human, Recombinant, E. coli

Granzyme B, Human, Recombinant, E. coli, is a serine protease used by activated cytotoxic T cells to induce apoptosis. Induces cell perforation and initiates proteolysis leading to cell death.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: GzmB

368043
  
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Overview

Replacement Information
Description
OverviewRecombinant, human granzyme B expressed in E. coli. Granzyme B (GzmB) is a serine protease that is used by activated cytotoxic T lymphocytes (CTL) to induce target cell apoptosis. It is involved in the perforation of target cells and initiation of proteolysis that leads the cell into the apoptosis pathway.
Catalogue Number368043
Brand Family Calbiochem®
SynonymsGzmB
References
ReferencesWilharm, E., et al. 1999. J. Biol. Chem. 274, 27331.
Harris, J. L., et al. 1998. J. Biol. Chem. 273, 27364.
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1 nmol Ac-IEPD-<i>p</i>NA per min 25° C, pH 8.0.
EC number3.4.21.79
FormLiquid
FormulationIn TBS, 50% glycerol.
Applications
ApplicationGranzyme B, Human, Recombinant, E. coli, is a serine protease used by activated cytotoxic T cells to induce apoptosis. Induces cell perforation and initiates proteolysis leading to cell death.
Biological Information
Specific Activity≥7.0 units/µg protein
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
368043 0

Documentation

Granzyme B, Human, Recombinant, E. coli SDS

Title

Safety Data Sheet (SDS) 

Granzyme B, Human, Recombinant, E. coli Certificates of Analysis

TitleLot Number
368043

References

Reference overview
Wilharm, E., et al. 1999. J. Biol. Chem. 274, 27331.
Harris, J. L., et al. 1998. J. Biol. Chem. 273, 27364.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision07-January-2011 RFH
SynonymsGzmB
DescriptionRecombinant, human granzyme B expressed in E. coli. Granzyme B (GzmB) is a serine protease that is involved in cytotoxic T lymphocyte-induced (CTL) cell death. It is reported to participate in the perforation of target cells and initiation of proteolysis that leads apoptosis. Similar to caspases, granzyme B preferentially cleaves substrate proteins with aspartic acid in the P1 position.
FormLiquid
FormulationIn TBS, 50% glycerol.
EC number3.4.21.79
Specific activity≥7.0 units/µg protein
Unit definitionOne unit is defined as the amount of enzyme that will hydrolyze 1 nmol Ac-IEPD-pNA per min 25° C, pH 8.0.
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesWilharm, E., et al. 1999. J. Biol. Chem. 274, 27331.
Harris, J. L., et al. 1998. J. Biol. Chem. 273, 27364.