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AP124B Goat Anti-Mouse IgG Antibody, biotin-SP conjugate

AP124B
2 mL  
Purchase on Sigma-Aldrich

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Overview

Description
Catalogue NumberAP124B
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionGoat Anti-Mouse IgG Antibody, biotin-SP conjugate
OverviewAffinity purified goat anti-mouse IgG, Biotin conjugated.
Background InformationImmunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Product Information
FormatBiotin
PresentationPRESENTATION: Lyophilized. Buffer = 0.02 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA, 0.1% sodium azide.

RECONSTITUTION:

Reconstitute with sterile distilled water to match the volume indicated on the vial label.
Quality LevelMQ100
Applications
ApplicationDetect Mouse IgG using this Goat anti-Mouse IgG Antibody, biotin-SP conjugate validated for use in ELISA & WB.
Key Applications
  • ELISA
  • Western Blotting
Application NotesEIA: 1:50,000-1:100,000

Western blots using enzyme-conjugated streptavidin: 1:10,000-1:100,000.

Immunohistochemistry: 1:500-1:5,000

Flow cytometry: 1:200-1:1,000

Fluorescence Immunohisto/cytochemistry: 1:200-1:1,000

Optimal working dilutions must be determined by end user.
Biological Information
HostGoat
Species Reactivity
  • Mouse
Antibody TypePolyclonal Antibody
Purification MethodImmunoAffinity Purified
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size2 mL
Global Trade Item Number
Catalogue Number GTIN
AP124B 08436037124044