Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

AP112B Goat Anti-Human IgG Antibody, biotin conjugate

This Goat anti-Human IgG Antibody, biotin conjugate is validated for use in ELISA, WB for the detection of Human IgG.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Species Reactivity: Human
AP112B
2 mL  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed
Description
Catalogue NumberAP112B
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionGoat Anti-Human IgG Antibody, biotin conjugate
Background InformationIgG is a monomeric immunoglobulin, consists of two heavy chains (gamma chains) and two light chains. Each molecule has two antigen binding sites. IgG is the most abundant immunoglobulin. It is approximately equally distributed in blood and in interstitial fluid, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, providing protection to the fetus in its first weeks of life before its own immune system has developed. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).
References
Product Information
FormatBiotin
PresentationPurified by immunoaffinity chromatography using antigen coupled to agarose beads. Lyophilized. Buffer is 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA, and 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Goat anti-Human IgG Antibody, biotin conjugate is validated for use in ELISA, WB for the detection of Human IgG.
Key Applications
  • ELISA
  • Western Blotting
Application NotesEIA: 1:20,000-1:400,000 using enzyme-streptavidin conjugate.
Western blots: 1:20,000-1:400,000 using enzyme-streptavidin conjugate.
Immunohistochemistry: 1:500-1:5,000 using enzyme-streptavidin conjugate.
Flow cytometry: 1:200-1:1,000.
Fluorescence Immunohisto/cytochemistry: 1:200-1:1,1000.

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenPrepared from purified human IgG.
HostGoat
SpecificityThe antibody reacts with the heavy chains of human IgG and with the light chains common to most human immunoglobulins.
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Purification MethodImmunoAffinity Purified
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain lyophilized product at 2°-8°C for up to 12 months from date of receipt. After reconstitution the product is stable for six weeks at 2°-8°C as an undiluted liquid. Reconstitute vial with 2 mL of distilled water. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of antibody (and buffer salts) will decrease to one-half of the original after the addition of glycerol and final working dilutions must be adjusted accordingly. Avoid repeated freeze thaw cycles.
Packaging Information
Material Size2 mL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AP112B 04053252478307

Documentation

Goat Anti-Human IgG Antibody, biotin conjugate SDS

Title

Safety Data Sheet (SDS) 

Goat Anti-Human IgG Antibody, biotin conjugate Certificates of Analysis

TitleLot Number
GOAT ANTI-HUMAN IgG BIOTIN CONJUGATED 2475585
GOAT ANTI-HUMAN IgG BIOTIN CONJUGATED SECONDARY ANTIBODY - 2117136 2117136
GOAT ANTI-HUMAN IgG BIOTIN CONJUGATED - 2492061 2492061
GOAT ANTI-HUMAN IgG BIOTIN CONJUGATED - 3174897 3174897
GOAT ANTI-HUMAN IgG BIOTIN CONJUGATED - 3212810 3212810
GOAT ANTI-HUMAN IgG BIOTIN CONJUGATED - 3385800 3385800
GOAT ANTI-HUMAN IgG BIOTIN CONJUGATED - 3427264 3427264
GOAT ANTI-HUMAN IgG BIOTIN CONJUGATED - 3464588 3464588
GOAT ANTI-HUMAN IgG BIOTIN CONJUGATED - 3488179 3488179
GOAT ANTI-HUMAN IgG BIOTIN CONJUGATED - 3574525 3574525
Show more

References

Reference overviewPub Med ID
Herpes simplex virus type-2 specific glycoprotein G-2 immunomagnetically captured from HEp-2 infected tissue culture extracts.
Charles R Clavet, Aaron B Margolin, Patrick M Regan
Journal of virological methods  119  121-8  2004

Show Abstract 		15158593 15158593