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AP112A Goat Anti-Human IgG Antibody, Alkaline Phosphatase conjugate

AP112A
1 mL  
Purchase on Sigma-Aldrich

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Overview

Replacement Information
Description
Catalogue NumberAP112A
ReplacesMABN1055
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionGoat Anti-Human IgG Antibody, Alkaline Phosphatase conjugate
OverviewAlkaline Phosphatase-conjugated Affinity Purified Goat anti-Human IgG (H+L)
References
Product Information
FormatAlkaline Phosphatase
PresentationLyophilized. Buffer = 0.01 M Tirs HCl, 0.25 M NaCl, pH 8.0 with

15 mg/mL BSA and 0.05% sodium azide.

RECONSTITUTION:

Reconstitute with sterile distilled water to match the volume indicated on the vial label. Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature.
Quality LevelMQ100
Applications
ApplicationDetect Human IgG using this Goat anti-Human IgG Antibody, Alkaline Phosphatase conjugate validated for use in ELISA & WB.
Key Applications
  • ELISA
  • Western Blotting
Application NotesEIA and Western blots: 1:5,000-1:50,000.

Immunohistochemistry: 1:1,000-1:2,000.

Optimal working dilutions must be determined by end user.
Biological Information
HostGoat
SpecificityBased on immunoelectrophoresis, the antibody reacts with the heavy chains on human IgG and with light chains common to most human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins, but the antibody may cross-react with immunoglobulins from other species.
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Purification MethodSerum
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. After dilution do not use for more than one day. For extended storage after reconstitution, add an equal volume of glycerol (ACS grade for better) to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.

WARNING:

For research use only; not for use as a diagnostic.
Packaging Information
Material Size1 mL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AP112A 04053252587771

Documentation

Goat Anti-Human IgG Antibody, Alkaline Phosphatase conjugate SDS

Title

Safety Data Sheet (SDS) 

Goat Anti-Human IgG Antibody, Alkaline Phosphatase conjugate Certificates of Analysis

TitleLot Number
GOAT ANTI HUMAN IgG (H+L) ALKALINE PHOSPHATASE CONJUGATED AFFINITY PURIFIED - 2424472 2424472
GOAT ANTI HUMAN IgG (H+L) -2689099 2689099
GOAT ANTI HUMAN IgG (H+L) -2767266 2767266
GOAT ANTI HUMAN IgG (H+L) ALKALINE PHOSPHATASE CONJUGATED - 2492223 2492223
GOAT ANTI HUMAN IgG (H+L) ALKALINE PHOSPHATASE CONJUGATED - 3330247 3330247
GOAT ANTI HUMAN IgG (H+L) ALKALINE PHOSPHATASE CONJUGATED - 3471986 3471986
GOAT ANTI HUMAN IgG (H+L) ALKALINE PHOSPHATASE CONJUGATED - 3497303 3497303
GOAT ANTI HUMAN IgG (H+L) ALKALINE PHOSPHATASE CONJUGATED - 3519874 3519874
GOAT ANTI HUMAN IgG (H+L) ALKALINE PHOSPHATASE CONJUGATED - 3751872 3751872
GOAT ANTI HUMAN IgG (H+L) ALKALINE PHOSPHATASE CONJUGATED - 3900365 3900365

Data Sheet

Title
GOAT ANTI HUMAN IgG (H+L) ALKALINE PHOSPHATASE CONJUGATED AFFINITY PURIFIED