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109204 Giemsa's azur eosin methylene blue solution

for microscopy

Giemsa's azur eosin methylene blue solution MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
109204
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
1092040100 Glass bottle 100 ml
1092040500 Plastic bottle 500 ml
1092041000 Plastic bottle 1 l
1092042500 Plastic bottle 2.5 l
Description
Catalogue Number109204
DescriptionGiemsa's azur-eosin-methylene blue solution
OverviewGiemsa’s Azure Eosin Methylene Blue solution - for microscopy, can be used for the staining of blood and bone marrow smears, paraffin sections and clinical-cytological specimens. Giemsa staining is one of the standard procedures in histology, demonstrating various cells with their morphological features better than with H&E stain. When used in hematological applications, Giemsa’s stain is frequently used in combination with other staining solutions, e. g. with May-Grünwald’s solution for Pappenheim (MGG) overview staining. This ready-to-use staining solution generally stains the nuclei red, based on the molecular interaction between the eosin Y dye and an Azure B-DNA complex. Both dyes assemble to an Eosin Y - Azure B-DNA complex and the intensity of the resulting stain depends on the content of Azure B and the ratio of Azure B : Eosin Y. Furthermore, the resulting stain can vary depending on the influence of fixation, staining times, pH-value of the solutions or buffer substances. In histology and clinico-cytological applications, Giemsa’s staining without additional stainings is used as an extended overview staining method. In this method, the color of the various cell components is influenced by pretreatment of the specimen material. Here, chromatin-containing structures (e. g. cell nuclei) appear in various blue shades, while the acidophilic components show up in a variety of red shades. The 500 ml bottle is sufficient for 3500-5000 stainings. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
References
Product Information
HS Code3212 90 90
Quality LevelMQ400
Applications
Applicationfor microscopy
Biological Information
Physicochemical Information
Boiling point>65 °C (1013 hPa)
Density0.99 g/cm3 (20 °C)
Explosion limit5.5 - 44 %(V) Methanol
Flash point18 °C
Ignition temperature455 °C
pH value6.0 - 8.2 (H₂O, 20 °C) (undiluted)
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Hazard Pictogram(s) Hazard Pictogram(s)
Hazard Pictogram(s)
Hazard Pictogram(s)
Hazard Statement(s)H225: Highly flammable liquid and vapour.
H301 + H311 + H331: Toxic if swallowed, in contact with skin or if inhaled.
H317: May cause an allergic skin reaction.
H370: Causes damage to organs.
Precautionary Statement(s)P210: Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
P233: Keep container tightly closed.
P280: Wear protective gloves/ protective clothing/ eye protection/ face protection/ hearing protection.
P301 + P310: IF SWALLOWED: Immediately call a POISON CENTER/doctor.
P303 + P361 + P353: IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water.
P304 + P340 + P311: IF INHALED: Remove person to fresh air and keep comfortable for breathing. Call a POISON CENTER/doctor.
P403 + P233: Store in a well-ventilated place. Keep container tightly closed.
Signal WordDanger
Storage class3 Flammable liquids
WGKWGK 2 obviously hazardous to water
Disposal9
Carcinogenic compounds and flammable compounds labelled "Highly toxic" or "Toxic": container F. Alkyl sulfates are carcinogenic; Take particular care to avoid inhalation and skin contact. To neutralize alkyl sulfates, add dropwise (from a dropping funnel) to concentrated ice-cool Ammonia solution (Cat.No. 105426) with vigorous stirring. Before placing in container D, check the pH with pH Universal indicator strips (Cat.No. 109535).
Safety Information
Categories of dangerhighly flammable, toxic
Product Usage Statements
Storage and Shipping Information
StorageStore at +15°C to +25°C.
Packaging Information
Transport Information
Declaration (railroad and road) ADR, RIDUN 1230 , 3 (6.1), II
Declaration (transport by air) IATA-DGRUN 1230 , 3 (6.1), II
Declaration (transport by sea) IMDG-CodeUN 1230 , 3 (6.1), II
Supplemental Information
Specifications
Suitability for microscopypasses test
Erythrocytesreddish-brownish
Nuclei / Chromatinred to violet
Eosinophilic granulesreddish to red-brown
Neutrophilic granuleslight violet
Lymphocyte cytoplasmnblue
Monocyte cytoplasmgrey-blue
Thrombocytesviolet
Basophilic granulesdark violet
Nuclei (Tissue section)blue to dark blue
Cellsblue to dark blue
Collagenpale blue
Osteoidpale blue
Eosinophilic granula (Tissue section)red
Acidic mucopolysaccharidesreddish-violet
Mast cell granulesreddish-violet
Cartilage matrixreddish-violet
Acidophilic materialsorange-red
Helicobacter pylori
blue to dark blue
blue to dark blue
Global Trade Item Number
Catalogue Number GTIN
1092040100 04022536111841
1092040500 04022536111889
1092041000 04022536720081
1092042500 04022536111926

Documentation

Giemsa's azur eosin methylene blue solution SDS

Title

Safety Data Sheet (SDS) 

Giemsa's azur eosin methylene blue solution Certificates of Analysis

Product Number PackagingSpecificationLot Number
1092040100 Glass bottlePDF Specification Document
1092040500 Plastic bottlePDF Specification Document
1092041000 Plastic bottlePDF Specification Document
1092042500 Plastic bottlePDF Specification Document

User Guides

Title
User Guide (IFU) - 109204

Related Products & Applications

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Catalogue Number Description
109203 Giemsa's azur-eosin-methylene blue

Accessories (Primary)

Catalogue Number Description
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Accessories (Secondary)

Catalogue Number Description
101424 May-Grünwald's eosine-methylene blue solution modified

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