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345845 GGTase Substrate, Fluorogenic

GGTase Substrate, Fluorogenic MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Dansyl-GCVLL, Dns-GCVLL

345845
  
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Overview

Replacement Information
Description
OverviewA pentapeptide based on the carboxyl terminus of H-Ras with a dansyl group attached to the amino terminus. Serves as a fluorescent substrate for continuously monitoring fluorescent protein-geranylgeranyltransferase (type I) (GGTase-I) activity (Km = 5 µM) in the presence of geranylgeranyl diphosphate. S-geranylgeranylation of the cysteine moiety results in a significant enhancement in fluorescence intensity (monitored at λ = 460 nm). Reported to be highly selective for GGTase and is not recognized by FTase.
Catalogue Number345845
Brand Family Calbiochem®
SynonymsDansyl-GCVLL, Dns-GCVLL
References
ReferencesClausen, V.A., et al. 2001. Biochemistry 40, 3920. Zhang, F.L., and Casey, P.J. 1996. Biochem. J. 320, 925. Pickett, W.C., et al. 1995. Anal. Biochem. 225, 60.
Product Information
FormLyophilized
FormulationSupplied as a trifluoroacetate salt.
Hill FormulaC₃₄H₅₂N₆O₈S₂
Chemical formulaC₃₄H₅₂N₆O₈S₂
Hygroscopic Hygroscopic
Structure formula ImageStructure formula Image
Applications
Biological Information
Purity≥95% by HPLC
Physicochemical Information
Emission max.
Excitation max.
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
345845 0

Documentation

GGTase Substrate, Fluorogenic Certificates of Analysis

TitleLot Number
345845

References

Reference overview
Clausen, V.A., et al. 2001. Biochemistry 40, 3920. Zhang, F.L., and Casey, P.J. 1996. Biochem. J. 320, 925. Pickett, W.C., et al. 1995. Anal. Biochem. 225, 60.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision12-December-2008 RFH
SynonymsDansyl-GCVLL, Dns-GCVLL
DescriptionA pentapeptide substrate based on the carboxyl terminus of H-Ras with a dansyl group attached to the amino terminus. Useful for continuous fluorescent monitoring of protein geranylgeranyltransferase (type I) (GGTase-I) activity in the presence of geranylgeranyl diphosphate (Km = 5 µM). S-geranylgeranylation of the cysteine residue results in a significant enhancement of fluorescence intensity at a wavelength of 460 nm. Reported to be highly specific for GGTase-I and is not recognized by protein farnesyltransferase (FTase). Excitation maximum: ~360 nm; emission maximum: ~460 nm.
FormLyophilized
FormulationSupplied as a trifluoroacetate salt.
Intert gas (Yes/No) Packaged under inert gas
Recommended reaction conditions10 µM geranylgeranyl diphosphate, 10 µM Dansyl-GCVLL, 50-200 ng GGTase-I in 50 mM Tris-HCl, pH 7.5, 50 mM ZnCl2, 5 mM MgCl2, 1 mM DTT, 20 mM KCl, and 0.2% octyl-β-D-glucopyranoside.
Chemical formulaC₃₄H₅₂N₆O₈S₂
Structure formulaStructure formula
Purity≥95% by HPLC
SolubilityDMSO (10 mg/ml)
Storage Protect from light
-20°C
Hygroscopic
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
Toxicity Standard Handling
ReferencesClausen, V.A., et al. 2001. Biochemistry 40, 3920. Zhang, F.L., and Casey, P.J. 1996. Biochem. J. 320, 925. Pickett, W.C., et al. 1995. Anal. Biochem. 225, 60.