Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

345810 G 418 Sulfate, Cell Culture Tested - CAS 108321-42-2 - Calbiochem

G418 also known as Geneticin® is an aminoglycoside antibiotic related to Gentamicin. Used as a selective agent in transfection of eukaryotic cells. Has highest potency ≥730 µg/mg and purity ≥98%.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: NMD Inhibitor I, G418, G 418

345810
  
Purchase on Sigma-Aldrich

Overview

Key Spec Table

CAS #Empirical Formula
108321-42-2C₂₀H₄₀N₄O₁₀ · 2H₂SO₄
Description
OverviewAminoglycoside related to gentamycin that inhibits prokaryotic and eukaryotic protein synthesis. Toxic to bacteria, yeast, protozoans, helminths, higher plant, and mammalian cells. Widely used in the selection of eukaryotic expression vectors carrying the bacterial neor/kanr genes. The product of these genes, aminoglycoside 3′-phosphotransferase, inactivates G418, neomycin, and kanamycin by phosphorylation. Introduction of either of these genes into cells can confer resistance to G418, which enables cells to grow in media containing G418.
Also available as a sterile-filtered aqueous solution (Cat. No. 345812), a sterile filtered solution in 100 mM HEPES (Cat. No. 508940), and a sterile-filtered solution PBS (Cat. No. 509227).
Catalogue Number345810
Brand Family Calbiochem®
SynonymsNMD Inhibitor I, G418, G 418
References
ReferencesEthier, S.P., and Taback, E. 1993. Cancer Lett. 74, 189.
Santerre, R.F., et al. 1991. Methods Mol. Biol. 7, 245.
Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol. 133, 46.
Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol. 163, 8.
Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA 79, 5166.
Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA 79, 7356.
Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun. 101, 1031.
Jimenez, A., and Davies, J. 1980. Nature 287, 869.
Product Information
CAS number108321-42-2
FormWhite powder
Hill FormulaC₂₀H₄₀N₄O₁₀ · 2H₂SO₄
Chemical formulaC₂₀H₄₀N₄O₁₀ · 2H₂SO₄
Structure formula ImageStructure formula Image
Quality LevelMQ100
Biological Information
Biological activityAssay: ≥730 µg/mg
Purity≥98% by TLC
Physicochemical Information
ContaminantsAmmonia: ≤1.0%; heavy metals: ≤100 ppm
Safety Information according to GHS
RTECSCB9378500
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +15°C to +30°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, sterilize by filtration through a 0.22 µm or 0.45 µm pore size filter, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Sterile stock solutions are stable for up to 1 year at -20°C.
Global Trade Item Number
Catalogue Number GTIN
345810 0