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QIA27 Fas Ligand ELISA Kit

Fas Ligand ELISA Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: CD95L ELISA Kit

Detection Methods: Colorimetric
QIA27
  
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Overview

Replacement Information

Key Spec Table

Detection Methods
Colorimetric
Description
Overview

This product has been discontinued.





Suitable for the in vitro quantitation of human FasL. Recognizes both membrane bound and soluble FasL.
Catalogue NumberQIA27
Brand Family Calbiochem®
SynonymsCD95L ELISA Kit
Materials Required but Not Delivered• 2-20 µl, 20-200 µl and 200-1000 µl precision pipetters with disposable tips.
• Automated plate washer, wash bottle or multichannel dispenser for washing.
• 1 liter graduated cylinder.
• Deionized or distilled H2O.
• 0.2 µm syringe filter and syringe.
• Spectrophotometer capable of measuring absorbance in 96-well plates using dual wavelength of 450/595 nm or 450/540 nm. A single wavelength of 450 nm can also be used.
• Cell Resuspension Buffer: 50 mM Tris, containing 5 mM EDTA, 0.2 mM PMSF, 1 µg/ml pepstatin, and 0.5 µg/ml leupeptin adjusted to pH 8.0.
References
Product Information
Detection methodColorimetric
Form96 Tests
Format96-well plate
Kit containsCoated 96-Well Plate, FasL Standard, Detector Antibody, 400X Conjugate, Conjugate Diluent, Substrate, Sample Diluent, Wash Concentrate, Antigen Extract Agent, Stop Solution, Plate Sealers, and a user protocol.
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Biological Information
Assay range325 pg/ml
Assay time4 h
Sample TypeCell lysates, tissue culture medium, serum, and plasma
Physicochemical Information
Sensitivity0.02 ng/ml
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 23/24/25-35-36/37/38-40-43-41

Toxic by inhalation, in contact with skin and if swallowed.
Causes severe burns.
Irritating to eyes, respiratory system and skin.
Limited evidence of a carcinogenic effect.
May cause sensitization by skin contact.
Risk of serious damage to eyes.
S PhraseS: 26-36/37/39-45

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing, gloves and eye/face protection.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Product Usage Statements
Intended useThe Calbiochem® Fas Ligand ELISA is a non-isotopic immunoassay for the in vitro quantitation of human FasL protein in cell lysates, tissue culture medium, serum and plasma.
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage -20°C
Storage ConditionsUpon receipt of the kit, store standards at -20°C. All other components may be stored at 4°C. Conjugate 400X should not be stored at 4°C for more than 4 months. Do not expose reagents to excessive light. Let kit reagents and samples come to room temperature before use.
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Kit containsCoated 96-Well Plate, FasL Standard, Detector Antibody, 400X Conjugate, Conjugate Diluent, Substrate, Sample Diluent, Wash Concentrate, Antigen Extract Agent, Stop Solution, Plate Sealers, and a user protocol.
Specifications
Global Trade Item Number
Catalogue Number GTIN
QIA27 0