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325902 FKBP12, GST-Fusion, Human, Recombinant, E. coli

FKBP12, GST-Fusion, Human, Recombinant, E. coli is a full-length protein that is fused to GST at the N-terminus and expressed in E.coli. FKBP12 is a cytosolic receptor for both rapamycin and FK506.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: FK506 binding protein 1A, 12 kDa, FKBP1A

325902
  
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Overview

Replacement Information
Description
OverviewFull-length recombinant, human FK506 binding protein 12-rapamycin associated protein 1 (FKBP12) (accession number = NP000792) fused to GST at the N-terminus and expressed in E.coli. FKBP12 is a cytosolic receptor for both rapamycin and FK506 that also serves as a natural cofactor for rapamycin's inhibition of mTOR. The molecular weight of 38,000 Da includes the GST moiety.
One vial is enough for inhibitor screening of four K-LISA™ mTOR Activity Kits (CBA055) (400 wells).Upon initial thawing, aliquot and store at -70° C.
Catalogue Number325902
Brand Family Calbiochem®
SynonymsFK506 binding protein 1A, 12 kDa, FKBP1A
Application Data
The activity of mTOR was assessed without inhibitor and in the presence of rapamycin alone (Cat. No. 553210), FKBP12 alone (Cat. No. 325902), FKBP12/rapamycin complex, and Wortmannin (Cat. No. 681675), using the K-LISA™ mTOR Activity Kit (Cat. No. CBA055) as outlined in the Detailed Protocol. The FKBP12/rapamaycin inhibitor complex was pre-assembled by mixing 62.5 µg/ml FKBP12 and 50 µM rapamycin on ice for 10 min. The mTOR Standard (50 µl) was incubated for 15 min on ice with FKBP12 (2 µl: final concentration 2.5 µg/ml), rapamycin (2 µl; final concentration 2 µM). FKPP12/rapamycin complex (final concentration 2.5 µg/ml, 2 µM rapamycin) or wortmannin (final concentration 10 µM). The kinase reaction was initiated by transferring each mixture to a corresponding well containing 2X kinase buffer and ATP. The results were assessed as outlined in the user protocol.
References
ReferencesJin, Y.J., et al. 1991. Proc Natl Acad Sci USA 88, 6677.
Product Information
FormLiquid
FormulationIn PBS, 10% glycerol, pH 7.2.
Quality LevelMQ100
Applications
ApplicationFKBP12, GST-Fusion, Human, Recombinant, E. coli is a full-length protein that is fused to GST at the N-terminus and expressed in E.coli. FKBP12 is a cytosolic receptor for both rapamycin and FK506.
Biological Information
Purity≥90% by SDS-PAGE
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
325902 0

Documentation

FKBP12, GST-Fusion, Human, Recombinant, E. coli SDS

Title

Safety Data Sheet (SDS) 

FKBP12, GST-Fusion, Human, Recombinant, E. coli Certificates of Analysis

TitleLot Number
325902

References

Reference overview
Jin, Y.J., et al. 1991. Proc Natl Acad Sci USA 88, 6677.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision22-June-2007 JSW
SynonymsFK506 binding protein 1A, 12 kDa, FKBP1A
Application Data
The activity of mTOR was assessed without inhibitor and in the presence of rapamycin alone (Cat. No. 553210), FKBP12 alone (Cat. No. 325902), FKBP12/rapamycin complex, and Wortmannin (Cat. No. 681675), using the K-LISA™ mTOR Activity Kit (Cat. No. CBA055) as outlined in the Detailed Protocol. The FKBP12/rapamaycin inhibitor complex was pre-assembled by mixing 62.5 µg/ml FKBP12 and 50 µM rapamycin on ice for 10 min. The mTOR Standard (50 µl) was incubated for 15 min on ice with FKBP12 (2 µl: final concentration 2.5 µg/ml), rapamycin (2 µl; final concentration 2 µM). FKPP12/rapamycin complex (final concentration 2.5 µg/ml, 2 µM rapamycin) or wortmannin (final concentration 10 µM). The kinase reaction was initiated by transferring each mixture to a corresponding well containing 2X kinase buffer and ATP. The results were assessed as outlined in the user protocol.
DescriptionFull-length recombinant, human FK506 binding protein 12-rapamycin associated protein 1 (FKBP12) (accession number = NP000792) fused to GST at the N-terminus and expressed in E.coli. FKBP12 is a cytosolic receptor for both rapamycin and FK506 that also serves as a natural cofactor for rapamycin's inhibition of mTOR. The molecular weight of 38,000 Da includes the GST moiety.
FormLiquid
FormulationIn PBS, 10% glycerol, pH 7.2.
Concentration Label Please refer to vial label for lot-specific concentration
Purity≥90% by SDS-PAGE
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesJin, Y.J., et al. 1991. Proc Natl Acad Sci USA 88, 6677.