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341208 Exoenzyme C3, Clostridium botulinum

Exoenzyme C3, Clostridium botulinum, CAS 58319-92-9, catalyzes the hydrolysis of NAD and, in the presence of Rho, catalyzes the ADP-ribosylation of Asn41 in Rho.

Exoenzyme C3, Clostridium botulinum MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
341208
  
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Overview

Replacement Information
Description
Overview

This product has been discontinued.



Catalyzes the hydrolysis of NAD and, in the presence of Rho, catalyzes the ADP-ribosylation of Asn41 in Rho. Inhibits the progression of the cell cycle from G1 to S phase.
Catalogue Number341208
Brand Family Calbiochem®
References
ReferencesUdagawa, T., and McIntyre, B.W. 1996. J. Biol. Chem. 271, 12542.
Yamamoto, M., et al. 1994. J. Dermatol. Sci. 8, 103.
Yamamoto, M., et al. 1993. Oncogene 8, 1449.
Popoff, A., et al. 1991. Infect. Immun. 59, 3673.
Morii, N., et al. 1988. J. Biol. Chem. 263, 12420.
Hartree, E.F. 1972. Anal. Biochem. 48, 422.
Weber, K., and Osborn, M. 1969. J. Biol. Chem. 244, 4406.
Product Information
CAS number58319-92-9
FormSolid
FormulationLyophilized from 20 mM Tris, 50 mM NaCl, 0.5% sucrose, 0.1% dextran, pH 7.5
Quality LevelMQ100
Applications
Biological Information
Purity≥90% by SDS-PAGE
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 37

Irritating to respiratory system.
S PhraseS: 36

Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Irritant
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 6 months at -70°C.
Canadian export regulations Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
341208 0

Documentation

Exoenzyme C3, Clostridium botulinum SDS

Title

Safety Data Sheet (SDS) 

Exoenzyme C3, Clostridium botulinum Certificates of Analysis

TitleLot Number
341208

References

Reference overview
Udagawa, T., and McIntyre, B.W. 1996. J. Biol. Chem. 271, 12542.
Yamamoto, M., et al. 1994. J. Dermatol. Sci. 8, 103.
Yamamoto, M., et al. 1993. Oncogene 8, 1449.
Popoff, A., et al. 1991. Infect. Immun. 59, 3673.
Morii, N., et al. 1988. J. Biol. Chem. 263, 12420.
Hartree, E.F. 1972. Anal. Biochem. 48, 422.
Weber, K., and Osborn, M. 1969. J. Biol. Chem. 244, 4406.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision08-June-2016 RB
DescriptionCatalyzes the hydrolysis of NAD. Can be used as a reagent for the detection of Rho proteins. Predominantly catalyzes the ADP ribosylation of the Asn41 amino acid residue in Rho. Treatment of cultured cells with exoenzyme C3 has been shown to induce ADP-ribosylation of RhoA protein and to reduce cell growth rate and saturation density. Its growth inhibitory effects appear to result from blockage at the G1 to S phase transition, causing the accumulation of cells in the G1 phase.
FormSolid
FormulationLyophilized from 20 mM Tris, 50 mM NaCl, 0.5% sucrose, 0.1% dextran, pH 7.5
CAS number58319-92-9
Purity≥90% by SDS-PAGE
SolubilityReconstitute with sterile distilled H₂O
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 6 months at -70°C.
Toxicity Irritant
ReferencesUdagawa, T., and McIntyre, B.W. 1996. J. Biol. Chem. 271, 12542.
Yamamoto, M., et al. 1994. J. Dermatol. Sci. 8, 103.
Yamamoto, M., et al. 1993. Oncogene 8, 1449.
Popoff, A., et al. 1991. Infect. Immun. 59, 3673.
Morii, N., et al. 1988. J. Biol. Chem. 263, 12420.
Hartree, E.F. 1972. Anal. Biochem. 48, 422.
Weber, K., and Osborn, M. 1969. J. Biol. Chem. 244, 4406.