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14-553 EphB2 Protein, active, 10 µg

Active, N-terminal His6-tagged recombinant human EphB2 residues 560-end, for use in Kinase Assays.

EphB2 Protein, active, 10 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-553
10 µg  Also available in 250 µg size --call for pricing and availability and reference catalog number 14-553M when ordering the 250 µg size.
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number14-553
Brand Family Upstate
Trade Name
  • Upstate
DescriptionEphB2 Protein, active, 10 µg
OverviewN-terminal His6-tagged recombinant human EphB2 residues 560-end
References
Product Information
Quality LevelMQ100
Applications
ApplicationActive, N-terminal His6-tagged recombinant human EphB2 residues 560-end, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceHuman EphB2 expressed by baculovirus in Sf21 insect cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryEphrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members.
Gene Symbol
  • EPHB2
  • EphB2
  • CAPB
  • EPHT3
  • Hek5
  • DRT
  • EPTH3
  • TYRO5
  • ERK
  • HEK5
  • MGC87492
  • PCBC
  • Tyro5
Protein TargetEphB2
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyTK
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P29323 # Receptor for members of the ephrin-B family. Acts as a tumor suppressor.
SIZE: 1055 amino acids; 117493 Da
SUBUNIT: The ligand-activated form interacts with multiple proteins, including GTPase-activating protein (RASGAP) through its SH2 domain. Binds RASGAP through the juxtamembrane tyrosines residues. Interacts with PRKCABP and GRIP1 (By similarity).
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Brain, heart, lung, kidney, placenta, pancreas, liver and skeletal muscle. Preferentially expressed in fetal brain.
DISEASE:SwissProt: P29323 # Defects in EPHB2 are involved in the progression of prostate cancer [MIM:176807]. & Defects in EPHB2 are involved in the development of prostate cancer metastasis to the brain [MIM:603688].
SIMILARITY: SwissProt: P29323 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily. & Contains 2 fibronectin type-III domains. & Contains 1 protein kinase domain. & Contains 1 SAM (sterile alpha motif) domain.
Molecular Weight52kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by phosphorylation of Poly (Glu4-Tyr) (4:1)
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C
Packaging Information
Material Size10 µg
Material PackageAlso available in 250 µg size --call for pricing and availability and reference catalog number 14-553M when ordering the 250 µg size.
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
14-553 04053252328121

Related Products & Applications

Alternative Packsize

Catalogue Number Description
14-553M EphB2 Protein, active, 250 µg

Product Families

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Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
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Categories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases