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324768 Endostatin, Human, Recombinant, Pichia pastoris

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
324768
  
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Overview

Replacement Information
Description
OverviewRecombinant, human endostatin expressed in Pichia pastoris. Endostatin is a ~20 kDa C-terminal fragment of collagen XVIII that acts as a potent inhibitor of angiogenesis and tumor growth in vitro and in vivo. Induces tyrosine phosphorylation of Shc (SH2 domain adapter protein) leading to apoptosis in endothelial cells.
Catalogue Number324768
Brand Family Calbiochem®
References
ReferencesDixelius, J., et al. 2000. Blood 95, 3403. Boehm, T., et al. 1999. Yeast 15, 563. Dhanabal, M., et al. 1999. Biochem. Biophys. Res. Commun. 258, 345. Dhanabal, M., et al. 1999. J. Biol. Chem. 274, 11721. Moulton, K.S., et al. 1999. Circulation 99, 1726. OʹReilly, M.S., et al. 1997. Cell 88, 277.
Product Information
DeclarationSold under license of U.S. Patents 6,764,995, 6,746,865 and corresponding patents.
FormLiquid
FormulationIn 66 mM sodium phosphate, 59 mM NaCl, 17 mM citric acid, pH 6.2.
Applications
Biological Information
Biological activity10 µg/ml endostatin will inhibit 50% tube formation in the presence of 10 ng/ml VEGF and 70% tube formation in the presence of 5 ng/ml bFGF
Purity≥95% by HPLC
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
ContaminantsEndotoxin: ≤ 0.50 EU/mg protein
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
324768 0

Documentation

Endostatin, Human, Recombinant, Pichia pastoris Certificates of Analysis

TitleLot Number
324768

References

Reference overview
Dixelius, J., et al. 2000. Blood 95, 3403. Boehm, T., et al. 1999. Yeast 15, 563. Dhanabal, M., et al. 1999. Biochem. Biophys. Res. Commun. 258, 345. Dhanabal, M., et al. 1999. J. Biol. Chem. 274, 11721. Moulton, K.S., et al. 1999. Circulation 99, 1726. OʹReilly, M.S., et al. 1997. Cell 88, 277.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision10-October-2007 JSW
DescriptionRecombinant, human endostatin expressed in Pichia pastoris. Endostatin is a ~20 kDa C-terminal fragment of collagen XVIII that acts as a potent inhibitor of angiogenesis and tumor growth in vitro and in vivo. Induces tyrosine phosphorylation of Shc (SH2 domain adapter protein) leading to apoptosis in endothelial cells.
FormLiquid
FormulationIn 66 mM sodium phosphate, 59 mM NaCl, 17 mM citric acid, pH 6.2.
Concentration Label Please refer to vial label for lot-specific concentration
Purity≥95% by HPLC
ContaminantsEndotoxin: ≤ 0.50 EU/mg protein
Biological activity10 µg/ml endostatin will inhibit 50% tube formation in the presence of 10 ng/ml VEGF and 70% tube formation in the presence of 5 ng/ml bFGF
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesDixelius, J., et al. 2000. Blood 95, 3403. Boehm, T., et al. 1999. Yeast 15, 563. Dhanabal, M., et al. 1999. Biochem. Biophys. Res. Commun. 258, 345. Dhanabal, M., et al. 1999. J. Biol. Chem. 274, 11721. Moulton, K.S., et al. 1999. Circulation 99, 1726. OʹReilly, M.S., et al. 1997. Cell 88, 277.