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ES-002-D EmbryoMax® 2X Freezing Medium for ES Cells, formulated with 20% DMSO & Fetal Bovine Serum

The EmbryoMax 2X Freezing Medium for ES Cells is formulated with 20% DMSO & Fetal Bovine Serum and available in a 50 mL format.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Stem Cell Culture
ES-002-D
50 mL  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Key Applications
Cult
Description
Catalogue NumberES-002-D
Trade Name
  • Embryomax
  • Specialty Media
DescriptionEmbryoMax® 2X Freezing Medium for ES Cells, formulated with 20% DMSO & Fetal Bovine Serum
OverviewThis unique formulation of cell culture freezing media is for cryopreservation of a broad spectrum of mammalian cells (see viability data). Freezing Media contain 10% of the active cryopreservation ingredient (either DMSO or Glycerol). The remaining 90% is a proprietary mixture of Calf and Fetal Bovine Serum. The reagents used in these products are certified to be virus and mycoplasma free.

The sera utilized in this medium is approved by the United States Department of Agriculture (USDA) to ensure that sera is free of diseases such as Bovine Spongiform Encephalopathy (BSE).
References
Product Information
Quality LevelMQ200
Applications
ApplicationThe EmbryoMax 2X Freezing Medium for ES Cells is formulated with 20% DMSO & Fetal Bovine Serum and available in a 50 mL format.
Key Applications
  • Stem Cell Culture
Application NotesPicking and freezing of ES cells:

Wash Plate with PBS. Pick colonies into 96 wll plate (U shaped wells) with 35 µl of trypsin / well. Pick roughly 48 colonies, incubate at 37 °C for 10 minutes. Use Multichannel pipet to break up colonies. Transfer directly into 96 well plate with feeders and 150 µl of ES medium (without selection drug). Feed every day with ES medium containing selection drug (150 µg /ml neomycin, or 50 µg /ml of hygromycin). After 2 days cells should be ready to be frozen. Wash with PBS add 35 µl of Trypsin, incubate at 37 °C for 10 minutes, add 65 µl of ES medium. Use multichannel pipet to disperse cells. Transfer 65 µl into replica plate (U shaped wells) containing 65 µl of cold 2 x ES freezing medium. Wrap with parafilm, place on dry ice for 20 minutes, and transfer to -80 °C.
Biological Information
Media FormLiquid
Stem Cell Type
  • Mouse Embryonic Stem Cells
  • Induced Pluripotent Stem Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -80°C up to 2 years
Packaging Information
Material Size50 mL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ES-002-D 04053252284519