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17-10078 EZ-Magna ChIP™ HT96 Chromatin Immunoprecipitation Kit

The EZ-Magna ChIP HT96 kit allows the performance of chromatin Immunoprecipitation in a 96-well plate-based format.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function
17-10078
96 assays  Kit capacity: 96 chromatin immunoprecipitation assays
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number17-10078
Brand Family Upstate
Trade Name
  • EZ-Magna ChIP
  • Upstate
DescriptionEZ-Magna ChIP™ HT96 Chromatin Immunoprecipitation Kit
OverviewThe EZ-Magna ChIP HT96 kit allows the performance of chromatin Immunoprecipitation in a 96-well plate-based format. This reliable streamlined approach works for both cells and tissues and can be performed using either a multi-channel pipette or an automated liquid handling instrument. Each kit includes a complete set of validated, quality controlled reagents, positive and negative control antibodies and validated primer set plus a detailed protocol.

Features & Benefits
  • Complete set of materials for up to 96 ChIP reactions
  • Protein A+G bead blend for ChIP with a broader range of antibodies than A or G alone
  • Low Chromatin requirements: 10, 000 to 100, 000 cells per reaction
  • Includes negative and positive control antibodies and control primer set to simplify validation of experimental procedure
  • Optimized streamlined protocol with only a single buffer for sonication, IP, or wash; and protocols for automated liquid handling systems
  • Protocols for using cells or tissues
  • Direct analysis of DNA without additional clean-up steps
  • Compatible with ChIPAb+ validated antibody and primer sets
Materials Required but Not DeliveredMagna Grip HT96™ Rack 96-well magnetic separation stand (Cat.# 17-10071) or similar 96-well magnetic rack.
References
Product Information
Components
  • Magna ChIP protein A/G magnetic beads
  • HT96 Nuclei Isolation Buffer
  • HT96 ChIP Buffer (Sonication/ChIP/Wash)
  • Low Stringency IP Wash Buffer
  • HT96 ChIP Elution Buffer
  • Proteinase K Solution
  • Protease Inhibitor Cocktail III
  • 10X Glycine
  • 10X PBS
  • 96 Well ChIP Plate
  • 96 Well Thermal Plate
  • Plate Seal
  • Strip Caps
  • Anti-Trimethyl-Histone H3 (Lys4) -positive control antibody
  • Normal Rabbit IgG -negative control antibody
  • GAPDH Primers - PCR positive control
PresentationTwo boxes containing all necessary reagents to perform 96 chromatin immunoprecipitation reactions in a 96 well plate format. Supplied buffers are sufficient to generate 24 individual chromatin preparation reactions using 15 cm plates of cultured cells or tissue samples.
Quality LevelMQ100
Applications
ApplicationThe EZ-Magna ChIP HT96 kit allows the performance of chromatin Immunoprecipitation in a 96-well plate-based format.
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsUpon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 1 year from date of shipment when stored as directed.
Packaging Information
Material Size96 assays
Material PackageKit capacity: 96 chromatin immunoprecipitation assays
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
17-10078 04053252012136

Documentation

Brochure

Title
An Introduction to Antibodies and Their Applications
Magna ChIP™ HiSens Kits
New Products: Volume 3, 2012

Technical Info

Title
96 well Template 2 Up
96 well Template Single
Magna ChIP™ protein A/G bead blends simplify the ChIP process and reduce background signals
White Paper - The Message in the Marks: Deciphering Cancer Epigenetics

Data Sheet

Title
Magna ChIP™ HT96 Kits for high throughput chromatin immunoprecipitation.

User Guides

Title
EZ-Magna ChIP™ HT96 (Cat. No. 17-10078)