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14-627M EGFR (L861Q) Protein, active, 250 µg

Active, N-terminal GST-tagged, recombinant human EGF Receptor, amino acids 696-end containing the mutation L861Q, for use in Enzyme Assays.

14-627M
250 µg  Also available in 10 μg size (cat#14-627) and in bulk (cat#14-627-K).
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number14-627M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionEGFR (L861Q) Protein, active, 250 µg
OverviewN-terminal GST-tagged, recombinant human EGF Receptor, amino acids 696-end containing the mutation L861Q
References
Product Information
Quality LevelMQ300
Applications
ApplicationActive, N-terminal GST-tagged, recombinant human EGF Receptor, amino acids 696-end containing the mutation L861Q, for use in Enzyme Assays.
Key Applications
  • Enzyme Assays
Biological Information
Sourcebaculovirus in Sf21 insect cells
Entrez Gene Number
Gene Symbol
  • EGFR
  • ERBB1
  • ERBB
  • mENA
Protein TargetEGFR
Purification MethodGlutathione-agarose
Target Sub-FamilyTK
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P00533 # Isoform 2/truncated isoform may act as an antagonist.
SIZE: 1210 amino acids; 134277 Da
SUBUNIT: Binds RIPK1. CBL interacts with the autophosphorylated C- terminal tail of the EGF receptor. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. The autophosphorylated form interacts with PIK3C2B, maybe indirectly. Interacts with PELP1.
SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted.
TISSUE SPECIFICITY: Expressed in placenta. Isoform 2 is also expressed in ovarian cancers.
PTM: Phosphorylation of Ser-695 is partial and occurs only if Thr- 693 is phosphorylated. & Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.DISEASE:SwissProt: P00533 # Defects in EGFR are associated with lung cancer [MIM:211980].
SIMILARITY: SwissProt: P00533 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain.
MISCELLANEOUS: Binding of EGF to the receptor leads to dimerization, internalization of the EGF-receptor complex, induction of the tyrosine kinase activity, stimulation of cell DNA synthesis, and cell proliferation.
Molecular Weight85.8kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by phosphorylation of 0.1mg/ml Poly (Glu4-Tyr)
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-627) and in bulk (cat#14-627-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
14-627M 04053252924446

Documentation

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Alternative Packsize

Catalogue Number Description
14-627 EGFR (L861Q) Protein, active, 10 µg

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Categories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases