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L2113-BC EDTA (Versen), 1 %

L2113-BC
  
Purchase on Sigma-Aldrich

Overview

Description
OverviewTrypsin/EDTA solutions (0.05 or 0.02% in PBS, without Ca2+, Mg2+) are used for passaging monolayer cultures. Trypsin affects the cell membrane and the cellular, and also the intercellular mucoproteins. Trypsin can be inhibited with the use of serum.
Specifications:

Functional Test: Pass
Activity Test: Pass
pH: 7.2-7.5
Osmolality: 0.27-0.30 Osm/kg H20
Color: Colorless
Appearance: Clear
Recommendation Instructions:

Recommendations Trypsin (1:250)/EDTA solutions:
Trypsin (1:250)/EDTA solutions (0.05/0.02 % in PBS without Ca2+, Mg2+) to passage monolayer cultures:
1. Wash cell culture (monolayer) using physiological saline (w/o Ca2+, Mg2+).
2. Apply a small quantity of Trypsin (1:250)/EDTA solution with a pipette to cell culture flask; a thin film is sufficient.
3. Incubate culture flask at +37°C for approx. 5 min.
4. Centrifuge cell suspension or transfer directly to a serum containing culture medium.

With higher Trypsin concentrations, it is recommended to trypsinate at +2-+8°C.
Catalogue NumberL2113-BC
Brand Family Calbiochem®
Product Information
Quality LevelMQ300
Storage and Shipping Information
Ship Code Blue Ice Only
Storage +2°C to +8°C
Do not freeze Ok to freeze
Global Trade Item Number
Catalogue Number GTIN
L2113-BC 0